Cell derived hierarchical assembly of a novel phosphophoryn-based biomaterial.
Cell derived hierarchical assembly of a novel phosphophoryn-based biomaterial.
复制标题
新型基于磷酸基的生物材料的细胞衍生分层组装。
DOI：
10.1159/000158571
复制
发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Sfeir,Charles
中科院分区：
文献类型：
--
作者：
Li,Jinhua;Olton,Dana;Lee,Donghyun;Kumta,PrashantN;Sfeir,Charles
磷酸蛋白（PP）是一种酸性磷蛋白，属于小整合素结合配体N-连接糖蛋白（SIBLING）蛋白家族。PP是高度磷酸化的，每分子约有200个磷酸盐，对钙具有高亲和力。本手稿的目的是证明，PP具有自组装的能力，当它在哺乳动物细胞中的钙的存在下过表达。我们的数据表明，当PP使用腺病毒过表达时，自组装发生在含有高钙浓度的内质网（ER）中。我们推测PP的高度磷酸化状态和酸性性质的物理化学性质在其在ER中的组装中起着重要作用。似乎当达到PP的临界浓度时，则有利于并促进组装。这种自组装可能是由于几个因素。(1)ER为这种现象的发生提供了理想的环境，因为ER环境通常促进聚集[Stevens和Argon：Semin Cell Dev Biol 1999; 10：443-454]。(2)除了PP的物理化学性质之外，未折叠的蛋白质反应也可能在这种自组装中发挥作用[Schroder和考夫曼：Mutat Res 2005; 569：29-63]。未折叠的蛋白质反应可被广谱损伤激活，导致蛋白质错误折叠并最终阻断蛋白质合成进展至高尔基体，导致蛋白质在ER中积累。总之，我们的数据表明，PP具有自组装的能力，在一个层次的方式。
Phosphophoryn (PP) is an acidic phosphoprotein belonging to the small integrin-bindingligand N-linked glycoprotein (SIBLING) protein family. PP is highly phosphorylated with approximately 200 phosphates per molecule and has a high affinity for calcium. The aim of this manuscript is to demonstrate that PP has the ability to self-assemble when it is overexpressed in a mammalian cell in the presence of calcium. Our data show that when PP is overexpressed using an adenovirus, the self-assembly occurs in the endoplasmic reticulum (ER) which contains high calcium concentration. We hypothesize that the physicochemical properties of the highly phosphorylated state and acidic nature of PP are playing an important role in its assembly in the ER. It appears that when a critical concentration of PP is reached, the assembly is then favored and facilitated. This self-assembly could be due to several factors.(1) The ER provides an ideal environment for this phenomenon to occur, since the ER environment usually promotes aggregation [Stevens and Argon: Semin Cell Dev Biol 1999; 10: 443–454].(2) In addition to PP’s physicochemical properties, the unfolded protein response could also be playing a role in this self-assembly [Schroder and Kaufman: Mutat Res 2005; 569: 29–63]. Unfolded protein response could be activated by a broad spectrum of insults that result in protein misfolding and ultimately blocking of the protein synthesis progression to the Golgi apparatus resulting in an accumulation of the protein in the ER. In summary, our data show that PP has the ability to self-assemble in a hierarchical manner.
登录
查看更多内容
百日咳博德特氏菌丝状血凝素和淋巴细胞增殖促进因子对小鼠的保护活性。
DOI：
10.1093/infdis/150.6.823
发表时间：
1984
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Masaaki Oda;J. Cowell;D. G. Burstyn;Charles R. Manciark
通讯作者：
Charles R. Manciark
DOI：
--
发表时间：
1984
期刊：
Journal of the American Medical Association (JAMA)
影响因子：
--
作者：
A. Hinman;J. Koplan
通讯作者：
J. Koplan
百日咳和百日咳疫苗。
影响因子：
14.6
作者：
R. Gold
通讯作者：
R. Gold
婴儿和幼儿对无细胞百日咳疫苗的临床和血清学反应。
影响因子：
--
作者：
E. Anderson;R. Belshe;J. Bartram;M. Gurwith;P. Hung;M. Levner;S. Vernon
通讯作者：
S. Vernon
DOI：
10.1097/00006454-198409000-00019
发表时间：
1984-09
期刊：
The Pediatric Infectious Disease Journal
影响因子：
--
作者：
M. Pittman
通讯作者：
M. Pittman