Active immunization with lipopolysaccharide Pseudomonas antigen for chronic Pseudomonas bronchopneumonia in guinea pigs.
Active immunization with lipopolysaccharide Pseudomonas antigen for chronic Pseudomonas bronchopneumonia in guinea pigs.
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脂多糖假单胞菌抗原主动免疫豚鼠慢性假单胞菌支气管肺炎。
DOI：
10.1172/jci110358
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Arnaut,MA
中科院分区：
文献类型：
--
作者：
Pennington,JE;Hickey,WF;Blackwood,LL;Arnaut,MA
铜绿假单胞菌的慢性呼吸道感染是囊性纤维化患者的主要临床问题。由于抗菌药物通常对根除这些感染无效，因此应考虑采取额外的治疗或预防措施。在这项研究中，实验豚鼠模型的慢性铜绿假单胞菌支气管肺炎，以确定是否主动免疫与脂多糖（LPS）铜绿假单胞菌抗原可能会有利地影响这种感染的过程。气管支气管滴入显微镜下的琼脂珠悬浮液，用活菌P浸渍，建立实验性肺炎。感染4 wk后，被动血凝假单胞菌抗体滴度的几何平均数（倒数）为185±1.3，肺内含有16.8±4 × 103假单胞菌菌落形成单位/ml肺匀浆。在感染前4周给予假单胞菌免疫，导致显著更高的被动血凝滴度（474±1.4;P< 0.05），肺组织中活假单胞菌数量减少（2.4±0.6 × 103;P< 0.01），肺组织病理学减少。相比之下，在肺部感染建立后2周向动物提供假单胞菌免疫，没有提供明显的益处。同样，用非假单胞菌LPS抗原（大肠杆菌J5疫苗）预防性免疫也不能提供保护。使用改良的Raji细胞测定法检测接种和感染豚鼠血清中的循环免疫复合物，没有证据表明主动免疫增加了感染豚鼠中循环免疫复合物的频率。结论是，用假单胞菌LPS抗原预防性免疫可能会对随后的支气管肺炎假单胞菌产生保护作用，但在建立感染期间进行免疫则无益。图片
Chronic respiratory infection withPseudomonas aeruginosais a leading clinical problem among patients with cystic fibrosis. Because antimicrobial agents are usually ineffective in eradicating these infections, additional therapeutic or prophylactic measures should be considered. In this study, an experimental guinea pig model of chronicPseudomonas aeruginosabronchopneumonia was utilized to determine whether active immunization with lipopolysaccharide (LPS)P. aeruginosaantigen may favorably influence the course of this infection. Experimental pneumonia was established by tracheobronchial instillation of suspensions of microscopic agar beads, which were impregnated with viableP. aeruginosa.After 4 wk of infection, the geometric mean (reciprocal) passive hemagglutinating Pseudomonas antibody titer was 185±1.3, and lungs contained 16.8±4 × 103colony-forming units Pseudomonas/ml of lung homogenate. Pseudomonas immunization, given prior to a 4-wk infection, resulted in significantly higher passive hemagglutinating titers (474±1.4;P< 0.05), lower numbers of viable Pseudomonas in lung tissues (2.4±0.6 × 103;P< 0.01), and reduced histopathology in lungs. In contrast, providing Pseudomonas immunization to animals 2 wk after pulmonary infection was established, offered no apparent benefit. Likewise, no protection was afforded by prophylactic immunization with a non-Pseudomonas LPS antigen (Escherichia coliJ5 vaccine). Using a Raji cell assay, modified to detect circulating immune complexes in vaccinated and infected guinea pig sera, there was no evidence that active immunization increased the frequency of circulating immune complexes in infected guinea pigs.It is concluded that prophylactic immunization with Pseudomonas LPS antigen may confer protection from subsequent Pseudomonas bronchopneumonia, but that immunization during established infection is not beneficial.Images
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调理性抗体增强人肺泡巨噬细胞对铜绿假单胞菌吞噬作用的特异性。
影响因子：
15.9
作者：
H. Reynolds;J. A. Kazmierowski;H H Newball
通讯作者：
H H Newball
用针对 UDP-gal 差向异构酶缺陷突变体的抗血清治疗无粒细胞动物中的大肠杆菌和克雷伯氏菌菌血症。
影响因子：
4.4
作者：
Elizabeth J. Ziegler;Herndon Douglas;James E. Sherman;Charles E. Davis;Abraham I. Braude
通讯作者：
Abraham I. Braude
人体对铜绿假单胞菌的免疫力。
DOI：
10.1093/infdis/126.3.257
发表时间：
1972
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Lowell S. Young;Donald Armstrong
通讯作者：
Donald Armstrong
影响因子：
3.1
作者：
S. Schwarzmann;J. Boring
通讯作者：
J. Boring
铜绿假单胞菌：囊性纤维化患者的免疫状态
影响因子：
3.1
作者：
R. Doggett;G. Harrison
通讯作者：
G. Harrison