Effects of physiological aging factor on bone tissue engineering repair based on fetal BMSCs.
Effects of physiological aging factor on bone tissue engineering repair based on fetal BMSCs.
复制标题
生理衰老因素对基于胎儿BMSCs的骨组织工程修复的影响
DOI：
10.1186/s12967-018-1686-1
复制
发表时间：
2018-11-23
影响因子：
7.4
通讯作者：
Zhang ZY
中科院分区：
文献类型：
--
作者：
Wu D;Wang Z;Zheng Z;Geng Y;Zhang Z;Li Q;Zhou Q;Cao Y;Zhang ZY
目前，世界上许多实验室和医院都在尝试和探索这种组织工程骨移植（TEBG）策略的临床转化。许多成功的骨组织工程（BTE）修复案例都是基于年轻个体。但关于TEBG策略在生理性老年个体中的有效性的研究很少。方法在大型动物模型水平上，研究衰老因素对胎儿tebg颅骨修复效果的影响。我们采用胎骨髓基质细胞（fetal marrow stromal cells, feal - bmscs）作为种子细胞，结合脱钙骨基质（decalcified bone matrix， DBM）支架，对老年山羊和幼年山羊颅骨缺损进行修复。通过显微ct和组织学检查比较老年山羊和年轻山羊的修复效果。结果胎体- tebg对6月龄山羊颅骨缺损的修复效果优于老年山羊；在老年山羊中，虽然没有完全修复，但feal - tebg修复的缺陷优于Control DBM支架修复的缺陷。结论衰老因素影响胎儿tebg的骨修复效果；BTE策略即使在老年人中仍然有效。老年状态的改善可促进老年个体BTE的修复作用。
BackgroundAt present, many laboratories and hospitals all over the world are attempting and exploring the clinical transformation of this tissue engineered bone graft (TEBG) strategy. Many successful cases of bone tissue engineering (BTE) repair were based on young individuals. But there are little studies about the effectiveness of TEBG strategy in physiological aged individuals.MethodsIn this research, we studied whether aging factor has influence on the skull repair effect of Fetal-TEBG, at the level of the large animal models. We used the fetal bone marrow stromal cells (Fetal-BMSCs) as the seed cells, combining the decalcified bone matrix (DBM) scaffolds, to repair the skull defects of the aged goats and the young goats. The repair effects on both aged goat and young goat were compared by Micro-CT and histology examination.ResultsThe skull defects of the young goats could be repaired better than that of the aged goats after 6 months by Fetal-TEBG; In the aged goats, although not completely repaired, the defects repaired by Fetal-TEBG was better than that repaired by the Control DBM scaffold.ConclusionsAging factor has impact on the bone repair effect of Fetal-TEBG; and the BTE strategy is still efficacious even in the aged individuals. The improvement of the aged state may promote the repair effect of the BTE in the aged individuals.
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DOI：
10.1002/jor.23845
发表时间：
2018-06
期刊：
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
影响因子：
--
作者：
Ball AN;Donahue SW;Wojda SJ;McIlwraith CW;Kawcak CE;Ehrhart N;Goodrich LR
通讯作者：
Goodrich LR
影响因子：
5.2
作者：
Yamada, Yoichi;Nakamura, Sayaka;Ito, Kenji;Umemura, Eri;Hara, Kenji;Nagasaka, Tetsuro;Abe, Akihiro;Baba, Shunsuke;Furuichi, Yasushi;Izumi, Yuichi;Klein, Ophir D.;Wakabayashi, Toshihiko
通讯作者：
Wakabayashi, Toshihiko
影响因子：
1.4
作者：
Sagar, Rachel;Walther-Jallow, Lilian;Westgren, Magnus
通讯作者：
Westgren, Magnus
影响因子：
--
作者：
Zhu, L;Liu, W;Cao, YL
通讯作者：
Cao, YL
在犬模型中用脂肪干细胞和珊瑚支架修复颅骨缺损
影响因子：
14
作者：
Cui, Lei;Liu, Bo;Cao, Yilin
通讯作者：
Cao, Yilin