Potential Misidentification of Natural Isomers and Mass-Analogs of Modified Nucleosides by Liquid Chromatography-Triple Quadrupole Mass Spectrometry.
Potential Misidentification of Natural Isomers and Mass-Analogs of Modified Nucleosides by Liquid Chromatography-Triple Quadrupole Mass Spectrometry.
复制标题
液相色谱-三重四极杆质谱法对天然异构体和修饰核苷的质量类似物的潜在错误鉴定
作者：
三重四极杆质谱联用液相色谱（LC-TQ-MS）可以检测和量化存在于各种类型RNA中的修饰核苷，并且越来越多地用于表转录组学。然而，由于TQ-MS的低分辨率和迄今已鉴定的许多自然修饰核苷的结构复杂性（>160），异构体和质量类似物的区分可能存在问题，并且经常被忽视。本研究采用LC-TQ-MS在三种不同的分析柱上分离分析了17种核苷标准品，并通过实例讨论了导致分析物错误鉴定的三个主要原因：结构异构体、质量类似物和同位素串扰。希望本文的综述和实例将有助于提高LC-TQ-MS鉴定修饰核苷的准确性。
Triple quadrupole mass spectrometry coupled to liquid chromatography (LC-TQ-MS) can detect and quantify modified nucleosides present in various types of RNA, and is being used increasingly in epitranscriptomics. However, due to the low resolution of TQ-MS and the structural complexity of the many naturally modified nucleosides identified to date (>160), the discrimination of isomers and mass-analogs can be problematic and is often overlooked. This study analyzes 17 nucleoside standards by LC-TQ-MS with separation on three different analytical columns and discusses, with examples, three major causes of analyte misidentification: structural isomers, mass-analogs, and isotopic crosstalk. It is hoped that this overview and practical examples will help to strengthen the accuracy of the identification of modified nucleosides by LC-TQ-MS.
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DOI：
10.1002/anie.202106215
发表时间：
2021-10-25
期刊：
Angewandte Chemie (International ed. in English)
影响因子：
--
作者：
Kaiser S;Byrne SR;Ammann G;Asadi Atoi P;Borland K;Brecheisen R;DeMott MS;Gehrke T;Hagelskamp F;Heiss M;Yoluç Y;Liu L;Zhang Q;Dedon PC;Cao B;Kellner S
通讯作者：
Kellner S
影响因子：
14.9
作者：
Kellner S;Ochel A;Thüring K;Spenkuch F;Neumann J;Sharma S;Entian KD;Schneider D;Helm M
通讯作者：
Helm M
影响因子：
5
作者：
Estevez M;Valesyan S;Jora M;Limbach PA;Addepalli B
通讯作者：
Addepalli B
影响因子：
16.6
作者：
Lin H;Miyauchi K;Harada T;Okita R;Takeshita E;Komaki H;Fujioka K;Yagasaki H;Goto YI;Yanaka K;Nakagawa S;Sakaguchi Y;Suzuki T
通讯作者：
Suzuki T
影响因子：
4.1
作者：
Nachtergaele, Sigrid;He, Chuan
通讯作者：
He, Chuan