pMAT: An open-source software suite for the analysis of fiber photometry data.
pMAT: An open-source software suite for the analysis of fiber photometry data.
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DOI：
10.1016/j.pbb.2020.173093
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发表时间：
2021-03
期刊：
影响因子：
--
通讯作者：
Barker DJ
中科院分区：
文献类型：
--
作者：
Bruno CA;O'Brien C;Bryant S;Mejaes JI;Estrin DJ;Pizzano C;Barker DJ
神经元记录新技术的发展和用于记录细胞活动和神经递质结合的新型传感器的开发共同开创了神经科学领域的新纪元。在这些新技术中，光纤光度法是一种技术，在这种技术中，植入的光纤被用来记录来自大宗组织中基因编码的荧光传感器的信号。纤维光度法因其成本效益、能够检测具有特定解剖或遗传特征的神经元的活动，以及使用这些高度模块化的系统来记录大脑浅层和深层结构中的一个或多个传感器或大脑部位的能力而被广泛采用。尽管有这些好处，但实验室采用这项技术的一个主要障碍是与光纤光度数据分析相关的陡峭的学习曲线。由于分析管道缺乏标准化，这一点变得更加复杂。在本文中，我们介绍了PMAT，这是一个“光度模块分析工具”，允许用户通过使用图形用户界面来完成常见的分析例程。该工具可以部署在MatLab中并由更高级的用户进行编辑，但也可以作为独立部署的开源应用程序使用。
The combined development of new technologies for neuronal recordings and the development of novel sensors for recording both cellular activity and neurotransmitter binding has ushered in a new era for the field of neuroscience. Among these new technologies is fiber photometry, a technique wherein an implanted fiber optic is used to record signals from genetically encoded fluorescent sensors in bulk tissue. Fiber photometry has been widely adapted due to its cost-effectiveness, ability to examine the activity of neurons with specific anatomical or genetic identities, and the ability to use these highly modular systems to record from one or more sensors or brain sites in both superficial and deep-brain structures. Despite these many benefits, one major hurdle for laboratories adopting this technique is the steep learning curve associated with the analysis of fiber photometry data. This has been further complicated by a lack of standardization in analysis pipelines. In the present communication, we present pMAT, a ‘photometry modular analysis tool’ that allows users to accomplish common analysis routines through the use of a graphical user interface. This tool can be deployed in MATLAB and edited by more advanced users, but is also available as an independently deployable, open-source application.
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影响因子：
48
作者：
Kim CK;Yang SJ;Pichamoorthy N;Young NP;Kauvar I;Jennings JH;Lerner TN;Berndt A;Lee SY;Ramakrishnan C;Davidson TJ;Inoue M;Bito H;Deisseroth K
通讯作者：
Deisseroth K
影响因子：
8.8
作者：
Root DH;Barker DJ;Estrin DJ;Miranda-Barrientos JA;Liu B;Zhang S;Wang HL;Vautier F;Ramakrishnan C;Kim YS;Fenno L;Deisseroth K;Morales M
通讯作者：
Morales M
Hitchhiker的伏安法指南：体内快速扫描循环伏安法的急性和慢性电极。
影响因子：
5
作者：
Rodeberg NT;Sandberg SG;Johnson JA;Phillips PE;Wightman RM
通讯作者：
Wightman RM
DOI：
10.1126/science.aat4422
发表时间：
2018-06-29
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Patriarchi T;Cho JR;Merten K;Howe MW;Marley A;Xiong WH;Folk RW;Broussard GJ;Liang R;Jang MJ;Zhong H;Dombeck D;von Zastrow M;Nimmerjahn A;Gradinaru V;Williams JT;Tian L
通讯作者：
Tian L
影响因子：
8.8
作者：
Barker, David J.;Miranda-Barrientos, Jorge;Morales, Marisela
通讯作者：
Morales, Marisela