Best practice recommendations for the measurement and interpretation of salivary proinflammatory cytokines in biobehavioral research.
Best practice recommendations for the measurement and interpretation of salivary proinflammatory cytokines in biobehavioral research.
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DOI：
10.1016/j.bbi.2020.09.009
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发表时间：
2021-01
期刊：
影响因子：
--
通讯作者：
Granger DA
中科院分区：
文献类型：
--
作者：
Riis JL;Ahmadi H;Hamilton KR;Hand T;Granger DA
尽管唾液炎性细胞因子整合到跨生物行为，心理，临床和健康相关学科的研究中，但关于生物标本收集，处理和储存实践的指导很少，这些实践最大限度地提高了唾液细胞因子数据的质量和有效性。此外，唾液细胞因子和口腔健康相关的措施之间的关联很少进行评估，并占口腔健康领域以外的研究。为了解决这些差距，我们研究了唾液白细胞介素-1 β（IL-1β），IL-6，IL-8和肿瘤坏死因子-α（TNF-α）对唾液样本采集技术和冷链管理程序变化的敏感性。使用从150名健康成年人收集的唾液样本子集，我们测量唾液IL-1β、IL-6、IL-8、TNF-α和其他口腔健康相关指数（即，血液污染[转铁蛋白]和唾液基质蛋白酶-8）。除了检查与样本采集技术和冷链管理程序相关的细胞因子水平的变化外，我们还评估了细胞因子浓度与其他口腔健康相关指标水平之间的关系。我们发现IL-1β、IL-6和IL-8对样本采集和冷链管理程序的变化比TNF-α更稳健，所有细胞因子均与其他口腔健康相关指标呈正相关。根据我们的研究结果，我们建议分析物特定的指导测量和解释唾液细胞因子浓度。
Despite the integration of salivary inflammatory cytokines into research across the biobehavioral, psychological, clinical, and health-related disciplines, there is little guidance regarding the biospecimen collection, handling, and storage practices that maximize the quality and validity of salivary cytokine data. Furthermore, associations between salivary cytokines and measures related to oral health are rarely assessed and accounted for in studies outside the oral health fields. To address these gaps, we examine the sensitivity of salivary interleukin-1β (IL-1β), IL-6, IL-8, and tumor necrosis factor-α (TNF-α) to changes in saliva sample collection technique and cold chain management procedures. Using subsets of saliva samples collected from 150 healthy adults, we measure salivary IL-1β, IL-6, IL-8, TNF-α, and other oral health-related indices (i.e., blood contamination [transferrin], and salivary matrixmallotprotienase-8). In addition to examining changes in cytokine levels associated with sample collection technique and cold chain management procedures, we assess relations between cytokine concentrations and levels of other oral health-related measures. We found that IL-1β, IL-6, and IL-8 were more robust to changes in sample collection and cold chain management procedures than TNF-α, and all cytokines were positively associated with other oral health-related measures. Based on our findings, we recommend analyte-specific guidance for measuring and interpreting salivary cytokine concentrations.
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