Comparative study of subseafloor microbial community structures in deeply buried coral fossils and sediment matrices from the challenger mound in the porcupine seabight.
Comparative study of subseafloor microbial community structures in deeply buried coral fossils and sediment matrices from the challenger mound in the porcupine seabight.
复制标题
DOI：
10.3389/fmicb.2011.00231
复制
发表时间：
2011
影响因子：
5.2
通讯作者：
Inagaki F
中科院分区：
文献类型：
--
作者：
Hoshino T;Morono Y;Terada T;Imachi H;Ferdelman TG;Inagaki F
海底沉积环境中蕴藏着极其多样化的微生物群落。然而，目前尚不清楚这些沉积物中深埋的化石是否在深层微生物栖息地中发挥了生态作用，或者这些化石中的微生物群落是否与周围沉积物基质中的微生物群落不同。在这里，我们比较了冷水珊瑚碳酸盐岩和粘土基质中海底微生物的群落结构。样品是在综合大洋钻探计划考察307期间从位于U1317孔A的豪猪海灯的挑战者丘陵上获得的。从位于海底4、20和105 米的珊瑚化石和周围的沉积基质中提取脱氧核糖核酸。用聚合酶链式反应扩增细菌和古生菌的16S rRNA基因，共分析了213,792个16S rRNA基因标记序列。在门的水平上，两个生境中的优势微生物组成包括变形杆菌、微藻、硝化螺旋藻、氯杆菌和在所研究的所有三个深度的其他克伦考古群(MCG)。然而，在属和/或种的水平上(相似性阈值97.0%)，群落组成有很大的不同，在珊瑚化石和粘土基质中分别有69-75%和46-57%的细菌和古菌系统类型不重叠。物种丰富度分析表明，细菌群落总体上比古细菌群落更具多样性，珊瑚化石的多样性得分低于沉积物基质。然而，在所有被检查的样品中，微生物群落的均匀度并没有显著差异。没有从珊瑚中获得真核DNA序列，如18S rRNA基因。研究结果表明，即使在相同或相似的深度，生境的沉积学特征也是影响深海海底沉积生境微生物多样性和群落结构的重要因素。
Subseafloor sedimentary environments harbor remarkably diverse microbial communities. However, it remains unknown if the deeply buried fossils in these sediments play ecological roles in deep microbial habitats, or whether the microbial communities inhabiting such fossils differ from those in the surrounding sediment matrix. Here we compare the community structures of subseafloor microbes in cold-water coral carbonates (Madrepora oculata and Lophelia pertusa) and the clay matrix. Samples were obtained from the Challenger Mound in the Porcupine Seabight at Site U1317 Hole A during the Integrated Ocean Drilling Program Expedition 307. DNA was extracted from coral fossils and the surrounding sedimentary matrix at 4, 20, and 105 m below the seafloor. 16S rRNA genes of Bacteria and Archaea were amplified by PCR, and a total of 213,792 16S rRNA gene-tagged sequences were analyzed. At the phylum level, dominant microbial components in both habitats consisted of Proteobacteria, Firmicutes, Nitrospirae, Chloroflexi, and Miscellaneous Crenarchaeota Group (MCG) at all three of the depths examined. However, at the genus and/or species level (similarity threshold 97.0%), the community compositions were found to be very different, with 69–75 and 46–57% of bacterial and archaeal phylotypes not overlapping in coral fossils and the clay matrix, respectively. Species richness analysis revealed that bacterial communities were generally more diverse than archaea, and that the diversity scores of coral fossils were lower than those in sediment matrix. However, the evenness of microbial communities was not significantly different in all the samples examined. No eukaryotic DNA sequences, such as 18S rRNA genes, were obtained from the corals. The findings suggested that, even at the same or similar depths, the sedimentological characteristics of a habitat are important factors affecting microbial diversity and community structure in deep subseafloor sedimentary habitats.
登录
查看更多内容
影响因子：
14.9
作者：
Pruesse E;Quast C;Knittel K;Fuchs BM;Ludwig W;Peplies J;Glöckner FO
通讯作者：
Glöckner FO
影响因子：
4.9
作者：
Beck, M.;Riedel, T.;Engelen, B.
通讯作者：
Engelen, B.
影响因子：
4.4
作者：
Webster, G;Parkes, RJ;Weightman, AJ
通讯作者：
Weightman, AJ
影响因子：
4.4
作者：
Schloss, Patrick D.;Westcott, Sarah L.;Weber, Carolyn F.
通讯作者：
Weber, Carolyn F.
影响因子：
14.9
作者：
Cole JR;Wang Q;Cardenas E;Fish J;Chai B;Farris RJ;Kulam-Syed-Mohideen AS;McGarrell DM;Marsh T;Garrity GM;Tiedje JM
通讯作者：
Tiedje JM