Neutrophils require SKAP2 for reactive oxygen species production following C-type lectin and Candida stimulation.
Neutrophils require SKAP2 for reactive oxygen species production following C-type lectin and Candida stimulation.
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中性粒细胞在c型凝集素和念珠菌刺激后需要SKAP2来产生活性氧。
DOI：
10.1016/j.isci.2021.102871
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发表时间：
2021-08-20
期刊：
影响因子：
5.8
通讯作者：
Mecsas J
中科院分区：
文献类型：
--
作者：
Nguyen GT;Xu S;Adams W;Leong JM;Bunnell SC;Mansour MK;Sykes DB;Mecsas J
将病原体识别转化为中性粒细胞有效炎症反应的信号级联对于宿主存活至关重要。SKAP 2是一种衔接蛋白，在中性粒细胞被整合素、甲酰肽受体刺激后产生活性氧（ROS）以及宿主防御革兰氏阴性细菌病原体肺炎克雷伯氏菌和假结核耶尔森氏菌所需。使用来自小鼠HoxB 8永生化祖细胞的中性粒细胞，我们表明中性粒细胞中的SKAP 2对于纯化的C型凝集素受体激动剂和真菌病原体光滑念珠菌和白色念珠菌的最大ROS应答以及光滑念珠菌的稳健杀伤至关重要。念珠菌感染后，整合素和Syk磷酸化的由内而外信号传导独立于SKAP 2发生。然而，在Skap 2 −/−中性粒细胞中，感染光滑念珠菌和肺炎克雷伯菌后，Pyk 2、ERK 1/2和p38磷酸化显著降低。这些数据表明SKAP 2在ROS产生和宿主防御中的重要性，除了抗菌免疫之外，还包括CLR和念珠菌属物种。SKAP-2调节C型凝集素刺激后的中性粒细胞ROS SKAP-2是光滑念珠菌刺激的中性粒细胞ROS和杀死念珠菌和克雷伯氏菌诱导的Pyk 2、pERK 1/2和p38激活所必需的。中性粒细胞需要SKAP-2来控制肺炎克雷伯氏菌感染生物科学;细胞生物学;免疫学;微生物学
Signaling cascades converting the recognition of pathogens to efficient inflammatory responses by neutrophils are critical for host survival. SKAP2, an adaptor protein, is required for reactive oxygen species (ROS) generation following neutrophil stimulation by integrins, formyl peptide receptors, and for host defense against the Gram-negative bacterial pathogens, Klebsiella pneumoniae and Yersinia pseudotuberculosis. Using neutrophils from murine HoxB8-immortalized progenitors, we show that SKAP2 in neutrophils is crucial for maximal ROS response to purified C-type lectin receptor agonists and to the fungal pathogens, Candida glabrata and Candida albicans, and for robust killing of C. glabrata. Inside-out signaling to integrin and Syk phosphorylation occurred independently of SKAP2 after Candida infection. However, Pyk2, ERK1/2, and p38 phosphorylation were significantly reduced after infection with C. glabrata and K. pneumoniae in Skap2−/− neutrophils. These data demonstrate the importance of SKAP2 in ROS generation and host defense beyond antibacterial immunity to include CLRs and Candida species. SKAP-2 regulates neutrophil ROS following C-type lectin stimulation SKAP-2 is required for C. glabrata-stimulated neutrophil ROS and killing Candida and Klebsiella-induced activation of Pyk2, pERK1/2, and p38 requires SKAP-2 Neutrophils require SKAP-2 to control K. pneumoniae infection Biological sciences; Cell biology; Immunology; Microbiology
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影响因子：
3.4
作者：
Brunke S;Hube B
通讯作者：
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Dectin-1介导β-葡聚糖的生物学作用。
DOI：
10.1084/jem.20021890
发表时间：
2003-05-05
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Brown GD;Herre J;Williams DL;Willment JA;Marshall AS;Gordon S
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Gordon S
影响因子：
30.8
作者：
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通讯作者：
Rich, Stephen S.
DOI：
10.1074/jbc.m117.799080
发表时间：
2017-08-11
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Feinberg H;Jégouzo SAF;Rex MJ;Drickamer K;Weis WI;Taylor ME
通讯作者：
Taylor ME
影响因子：
64.8
作者：
Dujon, B;Sherman, D;Souciet, JL
通讯作者：
Souciet, JL