The Benefits of Exogenous NO: Enhancing Arabidopsis to Resist Botrytis cinerea
The Benefits of Exogenous NO: Enhancing Arabidopsis to Resist Botrytis cinerea
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外源NO的好处：增强拟南芥抵抗灰霉病的能力
DOI：
10.4236/ajps.2011.23060
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发表时间：
2011-09
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影响因子：
--
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中科院分区：
文献类型：
--
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灰葡萄孢（Botrytis cinerea）是一种坏死营养型真菌病原体，其影响广泛的宿主，包括拟南芥。用一氧化氮（NO）供体硝普钠（SNP）预处理拟南芥叶片抑制灰葡萄孢（Botrytis cinerea）侵染。此外，在本研究中，应用于叶子的SNP剂量水平对病原体的发育没有直接的毒性影响。用荧光染料硝基蓝四唑（NBT）和3，3-二氨基联苯胺（DAB）对叶片材料进行染色，并采用荧光光度法研究了NO与活性氧化物（ROS）的关系。结果表明，外源NO抑制了病原菌与拟南芥互作过程中ROS的产生，尤其是H2 O2的产生。这种抑制活性氧化物爆发的作用与延迟侵染发展的作用相一致。研究了NO水平升高对抗氧化酶的影响。SNP处理和SNP预处理后接种病原菌的叶片中过氧化氢酶（CAT）和愈创木酚过氧化物酶（POD）活性均有不同程度的提高。然而，超氧化物歧化酶（SOD）的活性在叶片中的研究是不变的。H_2O_2含量的降低可能是由于POD和CAT活性的升高所致。我们的研究表明，NO可能会引发某些代谢反应，即增强酶活性，抑制H_2O_2，最终导致对B的抗性。灰霉病
Botrytis cinerea is a necrotrophic fungal pathogen that impacts a wide range of hosts, including Arabidopsis. Pretreatment with nitric oxide (NO) donor sodium nitroprusside (SNP) on Arabidopsis leaves suppressed Botrytis cinerea infection development. Additionally, in this study the dosage levels of SNP applied to the leaves had no direct, toxic impact on the development of the pathogen. The relationship between NO and reactive oxidant species (ROS) was studied by using both spectrophotometrical methods and staining leaf material with fluorescent dyes, nitro blue tetrazolium (NBT), and with 3,3-diaminobenzidine (DAB). The results showed that exogenous NO restrained the generation of ROS, especially H2O2, as the pathogen interacted with the Arabidopsis plant. And this inhibition of reactive oxidant burst coincided with delay infection development in NO-supplied leaves. The influence of elevated level of NO on antioxidant enzymes was investigated in this study. The activities of catalase (CAT) and guaiacol peroxidase (POD) were increased to different degrees in both: 1) SNP treated only leaves, and 2) SNP pretreated leaves that were subsequently inoculateted with pathogens. However, the activity of superoxide dismutase (SOD) was unchanged in the leaves studied. The decrease in H2O2 content probably resulted from the increase in activities of POD and CAT. Our study suggests that NO might trigger some metabolic reactions, i.e. enhanced enzyme activity that restrains H2O2 which ultimately results in resistance to B. cinerea infection.
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影响因子：
64.8
作者：
Delledonne, M;Xia, YJ;Lamb, C
通讯作者：
Lamb, C
影响因子：
3.5
作者：
Clark, D;Durner, J;Klessig, DF
通讯作者：
Klessig, DF
DOI：
10.1111/j.1365-313x.2004.02232.x
发表时间：
2004-11
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
P. Veronese;X. Chen;B. Bluhm;J. Salmeron;R. Dietrich;T. Mengiste
通讯作者：
P. Veronese;X. Chen;B. Bluhm;J. Salmeron;R. Dietrich;T. Mengiste
影响因子：
2.7
作者：
A. Tiedemann
通讯作者：
A. Tiedemann
影响因子：
7.4
作者：
MEHDY, MC
通讯作者：
MEHDY, MC