Lactic acid from vaginal microbiota enhances cervicovaginal epithelial barrier integrity by promoting tight junction protein expression.
Lactic acid from vaginal microbiota enhances cervicovaginal epithelial barrier integrity by promoting tight junction protein expression.
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DOI：
10.1186/s40168-022-01337-5
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发表时间：
2022-08-31
期刊：
影响因子：
15.5
通讯作者：
中科院分区：
文献类型：
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宫颈阴道微生物群以乳杆菌为主的女性。感染包括艾滋病毒在内的性传播感染的风险降低，但所涉及的生物学机制仍然不明确。在这里，我们对南非年轻女性 (n = 113) 的阴道拭子样本进行了宏蛋白质组学分析，并对宫颈阴道上皮细胞培养物进行了转录组学分析，以检查乳酸（宫颈阴道乳杆菌产生的代谢物）调节生殖器上皮屏障功能的能力。与乳酸菌缺乏的女性相比，阴道乳酸菌占主导地位的女性表现出更高丰度的细菌乳酸脱氢酶，这是一种负责乳酸产生的关键酶，与上皮屏障蛋白丰度的增加独立相关。乳酸的生理浓度增强上皮细胞培养屏障的完整性并增加细胞间连接分子的表达。这些发现揭示了阴道乳酸增强生殖器上皮屏障完整性的新能力，可能有助于防止性传播病原体的入侵。视频摘要 在线版本包含可在 10.1186/s40168-022-01337-5 获取的补充材料。
Women with a cervicovaginal microbiota dominated by Lactobacillus spp. are at reduced risk of acquiring sexually transmitted infections including HIV, but the biological mechanisms involved remain poorly defined. Here, we performed metaproteomics on vaginal swab samples from young South African women (n = 113) and transcriptomics analysis of cervicovaginal epithelial cell cultures to examine the ability of lactic acid, a metabolite produced by cervicovaginal lactobacilli, to modulate genital epithelial barrier function. Compared to women with Lactobacillus-depleted microbiota, women dominated by vaginal lactobacilli exhibit higher abundance of bacterial lactate dehydrogenase, a key enzyme responsible for lactic acid production, which is independently associated with an increased abundance of epithelial barrier proteins. Physiological concentrations of lactic acid enhance epithelial cell culture barrier integrity and increase intercellular junctional molecule expression. These findings reveal a novel ability of vaginal lactic acid to enhance genital epithelial barrier integrity that may help prevent invasion by sexually transmitted pathogens. Video abstract The online version contains supplementary material available at 10.1186/s40168-022-01337-5.
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DOI：
10.1086/657320
发表时间：
2010-12-15
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Brotman RM;Klebanoff MA;Nansel TR;Yu KF;Andrews WW;Zhang J;Schwebke JR
通讯作者：
Schwebke JR
影响因子：
5.2
作者：
Anton L;Sierra LJ;DeVine A;Barila G;Heiser L;Brown AG;Elovitz MA
通讯作者：
Elovitz MA
影响因子：
17.1
作者：
Gajer P;Brotman RM;Bai G;Sakamoto J;Schütte UM;Zhong X;Koenig SS;Fu L;Ma ZS;Zhou X;Abdo Z;Forney LJ;Ravel J
通讯作者：
Ravel J
影响因子：
3.1
作者：
Cherne, Michelle D.;Cole, Amy L.;Cole, Alexander M.
通讯作者：
Cole, Alexander M.
影响因子：
2.8
作者：
Fettweis, Jennifer M.;Brooks, J. Paul;Buck, Gregory A.
通讯作者：
Buck, Gregory A.