Novel concept microarray enabling PCR and multistep reactions through pipette-free aperture-to-aperture parallel transfer.
Novel concept microarray enabling PCR and multistep reactions through pipette-free aperture-to-aperture parallel transfer.
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DOI：
10.1186/1472-6750-10-71
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发表时间：
2010-10-06
影响因子：
3.5
通讯作者：
Nishigaki K
中科院分区：
文献类型：
--
作者：
Kinoshita Y;Tayama T;Kitamura K;Salimullah M;Uchida H;Suzuki M;Husimi Y;Nishigaki K
微阵列为基因组学分析的发展做出了贡献。然而，由于它主要依赖于表面反应，因此很难进行体反应和顺序多步反应。另一方面，流行的微孔板技术具有能够进行平行多步反应的优点，但由于操作困难，在增加孔数（目前最多为9600孔）和减少处理体积方面已经达到了极限。在这里，我们报告了一种新的微阵列技术，它使我们能够探索先进的应用，称为微阵列与可管理的体积（MMV）。该技术的本质是在无移液管的情况下，通过离心直接平行转移，避免了处理过程中的蒸发和吸附损失。通过MMV板及其配套设备和技术的开发，实现了多种条件（256种）的生成以及包括PCR和体外翻译反应在内的平行多步反应的实现。通过应用MMV技术搜索溶菌酶结晶条件和选择针对a β结合或组织蛋白酶e抑制的肽，证明了这一点。随着微阵列（MMV）技术的引入，亚μ l尺度的平行反应和多步反应成为可能。
The microarray has contributed to developing the omic analysis. However, as it depends basically on the surface reaction, it is hard to perform bulk reactions and sequential multistep reactions. On the other hand, the popular microplate technology, which has a great merit of being able to perform parallel multistep reactions, has come to its limit in increasing the number of wells (currently, up to 9600) and reducing the volume to deal with due to the difficulty in operations. Here, we report a novel microarray technology which enables us to explore advanced applications, termed microarray-with-manageable volumes (MMV). The technical essence is in the pipette-free direct parallel transfer from well to well performed by centrifugation, evading the evaporation and adsorption-losses during handling. By developing the MMV plate, accompanying devices and techniques, generation of multiple conditions (256 kinds) and performance of parallel multistep reactions, including PCR and in vitro translation reactions, have been made possible. These were demonstrated by applying the MMV technology to searching lysozyme-crystallizing conditions and selecting peptides aimed for Aβ-binding or cathepsin E-inhibition. With the introduction of a novel concept microarray (MMV) technology, parallel and multistep reactions in sub-μL scale have become possible.
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DOI：
10.1007/s005420100114
发表时间：
2002-01-01
期刊：
MICROSYSTEM TECHNOLOGIES
影响因子：
--
作者：
Gerlach, A;Knebel, G;Schaller, T
通讯作者：
Schaller, T
影响因子：
7
作者：
Nakane, J;Broemeling, D;Davis, RW
通讯作者：
Davis, RW
影响因子：
12.6
作者：
Nagai, H;Murakami, Y;Tamiya, E
通讯作者：
Tamiya, E
影响因子：
2.9
作者：
ATAKA, M;TANAKA, S
通讯作者：
TANAKA, S
DOI：
10.1093/protein/15.10.843
发表时间：
2002-10-01
期刊：
PROTEIN ENGINEERING
影响因子：
--
作者：
Kitamura, K;Kinoshita, Y;Nishigaki, K
通讯作者：
Nishigaki, K