Collagenase Impacts the Quantity and Quality of Native Mesenchymal Stem/Stromal Cells Derived during Processing of Umbilical Cord Tissue.
Collagenase Impacts the Quantity and Quality of Native Mesenchymal Stem/Stromal Cells Derived during Processing of Umbilical Cord Tissue.
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DOI：
10.1177/0963689717744787
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发表时间：
2018-01
影响因子：
3.3
通讯作者：
Cetrulo CL
中科院分区：
文献类型：
--
作者：
Taghizadeh RR;Cetrulo KJ;Cetrulo CL
酶通常用作从组织宿主中释放细胞的生物化学手段，用于体外研究和/或体内移植。然而，在从给定组织释放天然细胞的过程中，酶对细胞的生物学和功能性影响的了解很少。一个具体原因是，没有技术作为非酶对照来比较给定处理组织的基线生物学和功能。我们已经开发了一种无菌的一次性使用的一次性系统（称为细胞处理系统或AC：Px®），其允许使用机械手段在封闭的标准化系统中处理实体组织以释放细胞，而不需要和/或使用任何生物化学、酶消化。在这份报告中，我们首次直接比较了在胶原酶存在和不存在的情况下处理相同脐带组织（UCT）的细胞输出。在胶原酶的存在下，我们观察到平均约2.7倍的天然间充质干细胞/基质细胞（MSC）产量减少，MSC特异性标志物CD 90、CD 29、CD 105、CD 73、CD 44、CD 36、CD 49 b、CD 49 a、CD 146、CD 295和CD 166以及内皮标志物CD 31减少。这些数据直接表明，使用胶原酶处理UCT以释放细胞在数量和细胞表面标志物表达方面影响细胞回收，因此可能影响回收的天然细胞群的体内功能。
Enzymes are commonly used as a biochemical means to liberate cells from a host of tissues for use in in vitro studies and/or in vivo transplantations. However, very little understanding exists of the biological and functional effects that enzymes have on cells during the process of releasing the native cells from a given tissue. One specific reason for this is that no technology has existed as a nonenzymatic control to compare baseline biology and function for a given processed tissue. We have developed a sterile, onetime use, disposable system (referred to as the AuxoCell Processing System or AC:Px®) that allows for processing of solid tissue in a closed, standardized system using mechanical means to liberate cells without the need and/or use of any biochemical, enzymatic digestion. In this report, for the first time, we directly compare the cellular outputs derived from processing the same umbilical cord tissue (UCT) in the presence and absence of collagenase. In the presence of collagenase, we observed on average, approximately a 2.7-fold reduction in native mesenchymal stem/stromal cell (MSC) yields and a reduction in MSC-specific markers CD90, CD29, CD105, CD73, CD44, CD36, CD49b, CD49a, CD146, CD295, and CD166 and in endothelial marker CD31. These data directly exhibit that the use of collagenase to process UCT to release cells impacts cell recovery with respect to number and cell surface marker expression and, hence, could affect the in vivo function of the recovered native cellular population.
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影响因子：
6
作者：
Davies JE;Walker JT;Keating A
通讯作者：
Keating A
影响因子：
3.7
作者：
Liu YH;Vaghjiani V;Tee JY;To K;Cui P;Oh DY;Manuelpillai U;Toh BH;Chan J
通讯作者：
Chan J
影响因子：
20.3
作者：
Ng, Felicia;Boucher, Shayne;Tanavde, Vivek
通讯作者：
Tanavde, Vivek
影响因子：
2.2
作者：
Ford, AL;Foulcher, E;Sedgwick, JD
通讯作者：
Sedgwick, JD
影响因子：
2.2
作者：
Autengruber, A.;Gereke, M.;Bruder, D.
通讯作者：
Bruder, D.