A 3D printed mimetic composite for the treatment of growth plate injuries in a rabbit model.
A 3D printed mimetic composite for the treatment of growth plate injuries in a rabbit model.
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DOI：
10.1038/s41536-022-00256-1
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发表时间：
2022-10-19
影响因子：
7.2
通讯作者：
Payne, Karin A.
中科院分区：
文献类型：
--
作者：
Yu, Yangyi;Fischenich, Kristine M.;Schoonraad, Sarah A.;Weatherford, Shane;Uzcategui, Asais Camila;Eckstein, Kevin;Muralidharan, Archish;Crespo-Cuevas, Victor;Rodriguez-Fontan, Francisco;Killgore, Jason P.;Li, Guangheng;McLeod, Robert R.;Miller, Nancy Hadley;Ferguson, Virginia L.;Bryant, Stephanie J.;Payne, Karin A.
生长板损伤影响儿科人口可能会导致不必要的骨修复组织，导致异常骨伸长。临床治疗包括骨棒切除和植入间置材料，但成功是有限的，骨棒经常改革。没有任何治疗试图使生长板软骨再生。在此，我们开发了一种3D打印的模拟生长板复合材料，作为一种潜在的再生医学方法，目的是防止肢体长度差异和诱导软骨再生。采用聚乙二醇基树脂和数字光处理技术，3D打印了一个机械支撑结构，该结构填充了含有软骨形成线索的软性软骨模拟水凝胶。我们的仿生复合材料具有与家兔生长板相似的力学性能，并在体外诱导家兔间充质间质细胞成软骨分化。我们在兔生长板损伤模型中评估了其作为骨棒切除后再生植入材料的效果。x线成像监测肢体长度和胫骨平台角，显微计算机断层扫描评估骨形态，组织学表征形成的修复组织。我们的3D打印生长板模拟复合材料与未经处理的对照、仅模拟软骨水凝胶条件和脂肪移植相比，改善了胫骨延长。然而，在体内，3D打印的模拟生长板复合材料在组织学上没有显示软骨再生。尽管如此，该研究证明了3D打印仿生复合材料改善肢体延长的可行性，这是一项关键的功能结果，支持其作为生长板损伤治疗的进一步研究。
Growth plate injuries affecting the pediatric population may cause unwanted bony repair tissue that leads to abnormal bone elongation. Clinical treatment involves bony bar resection and implantation of an interpositional material, but success is limited and the bony bar often reforms. No treatment attempts to regenerate the growth plate cartilage. Herein we develop a 3D printed growth plate mimetic composite as a potential regenerative medicine approach with the goal of preventing limb length discrepancies and inducing cartilage regeneration. A poly(ethylene glycol)-based resin was used with digital light processing to 3D print a mechanical support structure infilled with a soft cartilage-mimetic hydrogel containing chondrogenic cues. Our biomimetic composite has similar mechanical properties to native rabbit growth plate and induced chondrogenic differentiation of rabbit mesenchymal stromal cells in vitro. We evaluated its efficacy as a regenerative interpositional material applied after bony bar resection in a rabbit model of growth plate injury. Radiographic imaging was used to monitor limb length and tibial plateau angle, microcomputed tomography assessed bone morphology, and histology characterized the repair tissue that formed. Our 3D printed growth plate mimetic composite resulted in improved tibial lengthening compared to an untreated control, cartilage-mimetic hydrogel only condition, and a fat graft. However, in vivo the 3D printed growth plate mimetic composite did not show cartilage regeneration within the construct histologically. Nevertheless, this study demonstrates the feasibility of a 3D printed biomimetic composite to improve limb lengthening, a key functional outcome, supporting its further investigation as a treatment for growth plate injuries.
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