A SNaPshot assay for the rapid and simple detection of hepatitis B virus genotypes.
A SNaPshot assay for the rapid and simple detection of hepatitis B virus genotypes.
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DOI：
10.3892/mmr.2014.2372
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发表时间：
2014-09
影响因子：
3.4
通讯作者：
Huang A
中科院分区：
文献类型：
--
作者：
Lai G;Zhang W;Tang H;Zhao T;Wei L;Tao Y;Wang Z;Huang A
一个简单的技术鉴定常见的基因型的B型肝炎病毒（HBV）仍有待确定。本研究旨在建立这样一种方法。对A-D基因型的4种质粒和123份HBV感染者的临床血清标本进行基因分型。当HBV基因型达到1 × 103 copies/ml或BC基因型混合样本达到5%水平时，HBV基因型可成功检出。血清标本中HBVDNA的检测下限为2.14×102 IU/ml。该方法的灵敏度和特异性均为100%，与测序和克隆法的一致性分别为90.24和100%。基因型B、C、BC、BD和BCD的频率分别为65.0%、23.6%、7.3%、3.3%和0.8%。与DNA测序方法相比，使用快速简单的SNaPshot方法检测混合感染的准确性也较高。本研究的结果表明，SNaPshot技术可以准确区分HBV基因型A-D，并能够很容易地应用于HBV预后和治疗的监测工具。
A simple technique for the identification of common genotypes of the hepatitis B virus (HBV) remains to be identified. The present study was conducted to establish such a methodology. Four plasmids of genotypes A-D and 123 clinical serum specimens of HBV-infected patients were genotyped. HBV genotypes would be detected successfully when the HBV genotype reached a viral load of 1 × 103 copies/ml or the BC genotype mixed samples reached a 5% level. The lower limit of detection of HBV DNA in serum specimens was determined to be 2.14×102 IU/ml. The assay sensitivity and specificity were 100% and the consistency was demonstrated to reach as high as 90.24 and 100% compared with that of the DNA sequencing and cloning. The frequencies of the genotypes B, C, BC, BD and BCD were found to be 65.0, 23.6, 7.3, 3.3 and 0.8%, respectively. The accuracy of detection of the mixed infections was also higher using the rapid and simple SNaPshot method compared with that achieved with the DNA sequencing methods. The results of the present study indicated that the SNaPshot technique accurately distinguishes the HBV genotypes A-D and is able to be readily applied as a monitoring tool in HBV prognosis and treatment.
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影响因子：
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