Isolation and characterization of a syncytium-inducing, macrophage/T-cell line-tropic human immunodeficiency virus type 1 isolate that readily infects chimpanzee cells in vitro and in vivo
Isolation and characterization of a syncytium-inducing, macrophage/T-cell line-tropic human immunodeficiency virus type 1 isolate that readily infects chimpanzee cells in vitro and in vivo
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一种合胞体诱导型巨噬细胞/T 细胞系嗜性人类免疫缺陷病毒 1 型分离株的分离和表征，该病毒易于在体外和体内感染黑猩猩细胞
DOI：
10.1128/jvi.69.7.4453-4462.1995
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发表时间：
1995
影响因子：
5.4
通讯作者：
Malcolm A. Martin
中科院分区：
文献类型：
--
作者：
R. Shibata;M. Hoggan;Christine Broscius;G. Englund;Theodore S. Theodore;A. Buckler;L. O. Arthur;Zimra R. Israel;Alan Schultz;H. Lane;Malcolm A. Martin
从艾滋病患者身上分离的新鲜人类免疫缺陷病毒1型（HIV-1）在黑猩猩外周血单个核细胞（PBMC）中的传染性进行了筛选，以确定能够在接种动物体内产生高病毒载量的菌株。获得的23个分离株中只有3个在黑猩猩细胞中具有传染性。在这三种病毒中，只有一种（HIV-1DH12）能够在所有25只黑猩猩的PBMC样本中引发有效感染。HIV-1DH12组织培养感染具有极快的复制动力学，深刻的细胞病变，以及对黑猩猩和人类PBMC，原代人巨噬细胞和几种人类t细胞系的嗜性。在给黑猩猩接种50 - 50%的组织培养感染剂量的HIV-1DH12后1周内发生感染；在第1、2和4周从血浆中恢复了无细胞病毒，并与淋巴结病的发展有关。初次感染期间和接种后6个月的病毒载量与hiv -1血清阳性个体报告的病毒载量相当。
Fresh human immunodeficiency virus type 1 (HIV-1) isolates from patients with AIDS were screened for infectivity in chimpanzee peripheral blood mononuclear cells (PBMC) to identify strains potentially able to generate high virus loads in an inoculated animal. Only 3 of 23 isolates obtained were infectious in chimpanzee cells. Of these three, only one (HIV-1DH12) was able to initiate a productive infection in PBMC samples from all 25 chimpanzees tested. HIV-1DH12 tissue culture infections were characterized by extremely rapid replication kinetics, profound cytopathicity, and tropism for chimp and human PBMC, primary human macrophage, and several human T-cell lines. An infection was established within 1 week of inoculating a chimpanzee with 50 50% tissue culture infective doses of HIV-1DH12; cell-free virus was recovered from the plasma at weeks 1, 2, and 4 and was associated with the development of lymphadenopathy. Virus loads during the primary infection and at 6 months postinoculation were comparable to those reported in HIV-1-seropositive individuals.
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来自受感染和未受感染黑猩猩的 CD8 细胞抑制人类免疫缺陷病毒复制。
影响因子：
4.3
作者：
Castro,BA;Walker,CM;Eichberg,JW;Levy,JA
通讯作者：
Levy,JA
影响因子：
56.9
作者：
ZHU, TF;MO, HM;HO, DD
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影响因子：
56.9
作者：
CHENGMAYER, C;SETO, D;LEVY, JA
通讯作者：
LEVY, JA
影响因子：
158.5
作者：
DAAR, ES;MOUDGIL, T;HO, DD
通讯作者：
HO, DD
影响因子：
11.8
作者：
FULTZ, PN
通讯作者：
FULTZ, PN