Mucosal vaccination with a recombinant Salmonella typhimurium expressing human papillomavirus type 16 (HPV16) L1 virus-like particles (VLPs) or HPV16 VLPs purified from insect cells inhibits the growth of HPV16-expressing tumor cells in mice.
Mucosal vaccination with a recombinant Salmonella typhimurium expressing human papillomavirus type 16 (HPV16) L1 virus-like particles (VLPs) or HPV16 VLPs purified from insect cells inhibits the growth of HPV16-expressing tumor cells in mice.
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使用表达人乳头瘤病毒 16 型 (HPV16) L1 病毒样颗粒 (VLP) 或从昆虫细胞纯化的 HPV16 VLP 的重组鼠伤寒沙门氏菌进行粘膜疫苗接种，可抑制小鼠中表达 HPV16 的肿瘤细胞的生长。
DOI：
10.1006/viro.2000.0717
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发表时间：
2001
期刊：
影响因子：
3.7
通讯作者：
Nardelli-Haefliger,D
中科院分区：
文献类型：
--
作者：
Revaz,V;Benyacoub,J;Kast,WM;Schiller,JT;DeGrandi,P;Nardelli-Haefliger,D
人类乳头瘤病毒，主要是16型（HPV16），是宫颈上皮内瘤变的罪魁祸首，这种瘤变与其他因素一起可导致宫颈癌。组装HPV16病毒样颗粒（VLPs）的沙门氏菌重组疫苗菌株和从昆虫细胞中纯化的HPV16病毒样颗粒都能在经鼻免疫的小鼠生殖器分泌物中诱导HPV16中和抗体。妇女宫颈分泌物中的抗HPV16特异性抗体可能预防HPV16生殖器感染，尽管在缺乏生殖器乳头瘤病毒感染的实验模型的情况下，这一点无法进行严格评估。在生殖器粘膜诱导hpv16特异性细胞介导的免疫可以提高疫苗的效力，而黏膜免疫途径可能是这样做的必要条件。研究表明，用纯化的HPV16 VLPs对小鼠进行全身免疫，可以通过诱导细胞毒性t淋巴细胞，保护小鼠免受表达HPV16的肿瘤细胞的攻击。使用相同的C3肿瘤模型，我们发现用纯化的HPV16 VLPs在预防环境下经鼻免疫小鼠也能诱导抗肿瘤免疫。更有趣的是，用稳定表达HPV16 L1 VLPs的沙门氏菌重组菌株接种小鼠粘膜疫苗，在预防和治疗环境中也能诱导抗肿瘤免疫。我们的数据表明，通过诱导中和抗体和细胞介导免疫，表达含有非结构病毒蛋白的嵌合VLPs的减毒沙门氏菌菌株可能是一种有希望的宫颈癌候选疫苗。
Human papillomaviruses, mainly type 16 (HPV16), are responsible for cervical intraepithelial neoplasia, which can lead, in association with other factors, to cervical cancer. Both Salmonella recombinant vaccine strains assembling HPV16 virus-like particles (VLPs) and HPV16 VLPs purified from insect cells are able to induce HPV16 neutralizing antibodies in genital secretions of mice after nasal immunization. Anti-HPV16-specific antibodies in cervical secretions of women may prevent genital infection with HPV16, although this cannot be critically evaluated in the absence of an experimental model for genital papillomavirus infection. Induction of HPV16-specific cell-mediated immunity in the genital mucosa could improve the efficacy of a vaccine and a mucosal route of immunization might be necessary to do so. It has been shown that systemic immunization of mice with purified HPV16 VLPs confers protection against an HPV16-expressing tumor cell challenge through the induction of cytotoxic T-lymphocytes. Using the same C3 tumor model, we show that intranasal immunization of mice with purified HPV16 VLPs in a prophylactic setting also induces anti-tumor immunity. More interestingly, mucosal vaccination of mice with a Salmonella recombinant strain stably expressing HPV16 L1 VLPs also induces anti-tumor immunity in prophylactic as well as in therapeutic settings. Our data suggest that attenuated Salmonella strains expressing chimeric VLPs containing nonstructural viral proteins might be a promising candidate vaccine against cervical cancer by inducing both neutralizing antibodies and cell-mediated immunity.
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使用沙门氏菌抗原递送系统产生细胞毒性 T 淋巴细胞反应
影响因子：
3.6
作者：
J. Flynn;W. Weiss;K. Norris;H. Seifert;S. Kumar;M. So
通讯作者：
M. So
DOI：
10.1099/0022-1317-75-8-2075
发表时间：
1994-08
期刊：
The Journal of general virology
影响因子：
--
作者：
R. Rose;R. Reichman;W. Bonnez
通讯作者：
R. Rose;R. Reichman;W. Bonnez
影响因子：
6.4
作者：
Gallichan, WS;Rosenthal, KL
通讯作者：
Rosenthal, KL
影响因子：
5.4
作者：
Dupuy, C;Buzoni-Gatel, D;Coursaget, P
通讯作者：
Coursaget, P
影响因子：
3.5
作者：
GALAN, JE;NAKAYAMA, K;CURTISS, R
通讯作者：
CURTISS, R