Nutritional Profile and Ecological Interactions of Yeast Symbionts Associated with North American Spruce Beetle (Dendroctonus rufipennis)
Nutritional Profile and Ecological Interactions of Yeast Symbionts Associated with North American Spruce Beetle (Dendroctonus rufipennis)
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与北美云杉甲虫 (Dendroctonus rufipennis) 相关的酵母共生体的营养状况和生态相互作用
DOI：
10.1007/s00248-022-02158-7
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发表时间：
2022
影响因子：
3.6
通讯作者：
Van Buiten, Charlene
中科院分区：
文献类型：
--
作者：
Davis, Thomas S.;Stewart, Jane E.;Clark, Caitlin;Van Buiten, Charlene
为了更好地了解树皮甲虫-微生物共生体的功能生态学，我们对北美云杉甲虫（Dendroctous rufipennisKirby）在不同种群中的酵母伙伴进行了表征。检测到七种酵母菌；Wickerhamomyces canadensis（Wickerham）Kurtzman 等人。 （Sachharomycetales：Saccharomycetaceae）是最常见的（占分离株的 74%），并且在所有种群中都有发现。 W. 分离株随后测试了 canadensis 与共生（Leptographium abietinum，= Grosmannia abietina）和致病性（Beauvaria bassiana）丝状真菌的竞争性相互作用，并对分离株进行了营养分析（蛋白质和磷含量）。暴露于酵母顶空排放物对共生真菌和病原真菌的菌落生长具有分离依赖性影响； W的大多数分离株。 canadensis 轻微抑制共生真菌（L. abietinum，平均效果： − 4%）和昆虫病原真菌（B. bassiana，平均效果： − 6%）的生长速率。然而，总体变异很大（范围： − 35.4 到 + 88.6%），一些酵母促进丝状真菌的生长，而其他酵母则始终抑制。挥发性2-苯基乙醇是由W.生产的。加拿大和合成的 2-苯基乙醇降低了两者的生长速度。松柏和B. bassiana平均为36%。 Wickerhamomyces canadensis培养物的平均蛋白质和磷含量分别为0.8%和7.2%，但分离株的营养成分不同，蛋白质含量与寄主树韧皮部相似。我们的结论是W. canadensis 是 D 的主要酵母共生体。 rufipennisin 在落基山脉中，并释放出可影响相关微生物生长的挥发物。Wickerhamomyces canadensisisolates 在限制营养素（蛋白质和磷）方面差异很大，但浓度低于共生丝状真菌报道的浓度。松香。
To better understand functional ecology of bark beetle-microbial symbioses, we characterized yeast associates of North American spruce beetle (Dendroctous rufipennisKirby) across populations. Seven yeast species were detected;Wickerhamomyces canadensis(Wickerham) Kurtzman et al. (Sachharomycetales: Saccharomycetaceae) was the most common (74% of isolates) and found in all populations. Isolates ofW. canadensiswere subsequently tested for competitive interactions with symbiotic (Leptographium abietinum, =Grosmannia abietina) and pathogenic (Beauvaria bassiana) filamentous fungi, and isolates were nutritionally profiled (protein and P content). Exposure to yeast headspace emissions had isolate-dependent effects on colony growth of symbiotic and pathogenic fungi; most isolates ofW. canadensisslightly inhibited growth rates of symbiotic (L. abietinum, mean effect: − 4%) and entomopathogenic (B. bassiana, mean effect: − 6%) fungi. However, overall variation was high (range: − 35.4 to + 88.6%) and some yeasts enhanced growth of filamentous fungi whereas others were consistently inhibitory. The volatile 2-phenylethanol was produced byW. canadensisand synthetic 2-phenylethanol reduced growth rates of bothL. abietinumandB. bassianaby 36% on average. Mean protein and P content ofWickerhamomyces canadensiscultures were 0.8% and 7.2%, respectively, but isolates varied in nutritional content and protein content was similar to that of host tree phloem. We conclude thatW. canadensisis a primary yeast symbiont ofD. rufipennisin the Rocky Mountains and emits volatiles that can affect growth of associated microbes.Wickerhamomyces canadensisisolates vary substantially in limiting nutrients (protein and P), but concentrations are less than reported for the symbiotic filamentous fungusL. abietinum.
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影响因子：
4.8
作者：
Herrera, Carlos M.;Pozo, Maria I.;Medrano, Monica
通讯作者：
Medrano, Monica
影响因子：
3.6
作者：
Davis, Thomas Seth;Boundy-Mills, Kyria;Landolt, Peter J.
通讯作者：
Landolt, Peter J.
云杉对树皮甲虫真菌共生体的本构防御和诱导防御的种间变异提供了对与抗性相关的性状的深入了解。
影响因子：
4
作者：
D. Ott;T. S. Davis;Javier E. Mercado
通讯作者：
Javier E. Mercado
关于昆虫-真菌共生中的相互作用、关联、菌体、共生体和共生体
影响因子：
2.9
作者：
F. Vega;P. H. Biedermann
通讯作者：
P. H. Biedermann
影响因子：
3.6
作者：
Davis, Thomas S.;Hofstetter, Richard W.;Keim, Paul
通讯作者：
Keim, Paul