Adolescent social housing protects against adult emotional and cognitive deficits and alters the PFC and NAc transcriptome in male and female C57BL/6J mice.
Adolescent social housing protects against adult emotional and cognitive deficits and alters the PFC and NAc transcriptome in male and female C57BL/6J mice.
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DOI：
10.3389/fnins.2023.1287584
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发表时间：
2023
影响因子：
4.3
通讯作者：
Wolstenholme JT
中科院分区：
文献类型：
--
作者：
Lodha J;Brocato ER;Nash M;Marcus MM;Pais AC;Pais AB;Miles MF;Wolstenholme JT
青春期是认知和情感发展的关键时期，其特征是高水平的社会互动和冒险行为的增加，包括酗酒。青少年暴露于社会压力和酗酒与社会，情感和认知缺陷的发展，以及酒精使用障碍的风险增加。早期生活中的社会压力和/或酗酒可能会破坏大脑皮层的发育，部分原因是这些持久的情绪，认知和行为影响。本研究的目的是实施一种新的邻居住房环境，以确定青少年邻居住房和/或酗酒对（1）一系列情感和认知任务（2）成人饮酒行为，以及（3）丘脑核和前额叶皮层转录组的影响。将青春期雄性和雌性C57 BL/6 J小鼠单独饲养或相邻饲养，可使用或不使用间歇性乙醇。一个队列在成年期进行了行为测试，以确定社会偏好，焦虑样行为的表达，认知能力和乙醇摄入模式。第二组在青春期后期处死，脑组织用于转录组学分析。作为成年人，单独圈养的小鼠显示出减少的社会互动，在新的物体识别任务的赤字，并增加焦虑样行为，相对于邻居圈养的小鼠。居住条件对青少年或成年人的乙醇消费量没有影响。青少年乙醇暴露不会改变成人乙醇摄入量。转录组学分析表明，青少年的住房条件和乙醇暴露导致差异表达的基因相关的突触可塑性的核和基因相关的甲基化，细胞外基质和炎症在前额叶皮层。行为的结果表明，在青春期通过邻居住房模式的社会交往可以防止情感，社会和认知缺陷。此外，转录组学结果表明，这些行为改变可能部分介导的额叶皮层或丘脑核转录失调。
Adolescence is a critical period in cognitive and emotional development, characterized by high levels of social interaction and increases in risk-taking behavior including binge drinking. Adolescent exposure to social stress and binge ethanol have individually been associated with the development of social, emotional, and cognitive deficits, as well as increased risk for alcohol use disorder. Disruption of cortical development by early life social stress and/or binge drinking may partly underlie these enduring emotional, cognitive, and behavioral effects. The study goal is to implement a novel neighbor housing environment to identify the effects of adolescent neighbor housing and/or binge ethanol drinking on (1) a battery of emotional and cognitive tasks (2) adult ethanol drinking behavior, and (3) the nucleus accumbens and prefrontal cortex transcriptome. Adolescent male and female C57BL/6J mice were single or neighbor housed with or without access to intermittent ethanol. One cohort underwent behavioral testing during adulthood to determine social preference, expression of anxiety-like behavior, cognitive performance, and patterns of ethanol intake. The second cohort was sacrificed in late adolescence and brain tissue was used for transcriptomics analysis. As adults, single housed mice displayed decreased social interaction, deficits in the novel object recognition task, and increased anxiety-like behavior, relative to neighbor-housed mice. There was no effect of housing condition on adolescent or adult ethanol consumption. Adolescent ethanol exposure did not alter adult ethanol intake. Transcriptomics analysis revealed that adolescent housing condition and ethanol exposure resulted in differential expression of genes related to synaptic plasticity in the nucleus accumbens and genes related to methylation, the extracellular matrix and inflammation in the prefrontal cortex. The behavioral results indicate that social interaction during adolescence via the neighbor housing model may protect against emotional, social, and cognitive deficits. In addition, the transcriptomics results suggest that these behavioral alterations may be mediated in part by dysregulation of transcription in the frontal cortex or the nucleus accumbens.
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影响因子：
--
作者：
Doremus-Fitzwater, T. L.;Deak, T.
通讯作者：
Deak, T.
影响因子：
3.5
作者：
Butler TR;Ariwodola OJ;Weiner JL
通讯作者：
Weiner JL
影响因子：
14.9
作者：
Chen J;Bardes EE;Aronow BJ;Jegga AG
通讯作者：
Jegga AG
DOI：
10.1111/acer.12586
发表时间：
2014-12
期刊：
Alcoholism, clinical and experimental research
影响因子：
--
作者：
Palm S;Nylander I
通讯作者：
Nylander I
DOI：
10.1097/01.alc.0000179368.87886.76
发表时间：
2005-09-01
期刊：
Alcoholism, clinical and experimental research
影响因子：
--
作者：
De Bellis, Michael D;Narasimhan, Anandhi;Clark, Duncan B
通讯作者：
Clark, Duncan B