A Low-Cost Palmtop High-Speed Capillary Electrophoresis Bioanalyzer with Laser Induced Fluorescence Detection.
A Low-Cost Palmtop High-Speed Capillary Electrophoresis Bioanalyzer with Laser Induced Fluorescence Detection.
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具有激光诱导荧光检测功能的低成本掌上高速毛细管电泳生物分析仪
DOI：
10.1038/s41598-018-20058-0
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发表时间：
2018-01-29
影响因子：
4.6
通讯作者：
Fang Q
中科院分区：
文献类型：
--
作者：
Pan JZ;Fang P;Fang XX;Hu TT;Fang J;Fang Q
在这项工作中，我们开发了一种集成全模块的小型化掌上高速毛细管电泳（CE）系统，包括皮升级进样、短毛细管快速毛细管电泳、高压电源、正交激光诱导荧光（LIF）检测、电池、系统控制、在线数据采集、处理、存储和显示模块。采用最小系统设计和低成本系统构建相结合的极简小型化策略，以极低的成本实现仪器小型化，这与目前大多数报道的小型化CE系统中使用的微加工策略不同。通过这样的策略，生物分析仪的总尺寸最小化至90 × 75 × 77 mm（长 × 宽度 × 高度），并且仪器成本降低至约1000万美元。 500 美元，展示了迄今为止报道的系统中具有 LIF 检测功能的最小且成本最低的 CE 仪器。本生物分析仪还表现出与之前报道的高速毛细管电泳系统相当的分析性能。获得了 1.02nM 荧光素钠的检测限。实现了氨基酸、氨基酸对映体、DNA片段、蛋白质等多种样品的高效快速分离。我们将该仪器应用于结直肠癌诊断中，通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法检测KRAS突变状态。
In this work, we developed a miniaturized palmtop high-speed capillary electrophoresis (CE) system integrating whole modules, including picoliter-scale sample injection, short capillary-based fast CE, high-voltage power supply, orthogonal laser induced fluorescence (LIF) detection, battery, system control, on-line data acquisition, processing, storage, and display modules. A strategy of minimalist miniaturization combining minimal system design and low-cost system construction was adopted to achieve the instrument miniaturization with extremely low cost, which is differing from the current microfabrication strategy used in most reported miniaturized CE systems. With such a strategy, the total size of the bioanalyzer was minimized to 90 × 75 × 77 mm (length × width × height) and the instrument cost was reduced to ca. $500, which demonstrated the smallest and lowest-cost CE instrument with LIF detection in so far reported systems. The present bioanalyzer also exhibited comparable analytical performances to previously-reported high-speed CE systems. A limit of detection of 1.02 nM sodium fluorescein was obtained. Fast separations were achieved for multiple types of samples as amino acids, amino acid enantiomers, DNA fragments, and proteins with high efficiency. We applied this instrument in colorectal cancer diagnosis for detecting KRAS mutation status by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.
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影响因子：
7.4
作者：
Fister, JC;Jacobson, SC;Ramsey, JM
通讯作者：
Ramsey, JM
影响因子：
4.1
作者：
Kawazumi, H;Song, JM;Ogawa, T
通讯作者：
Ogawa, T
影响因子：
7.4
作者：
Ermakov, SV;Jacobson, SC;Ramsey, JM
通讯作者：
Ramsey, JM
影响因子：
7.4
作者：
Fu, Jing-Lin;Fang, Qun;Fang, Zhao-Lun
通讯作者：
Fang, Zhao-Lun
影响因子：
2.9
作者：
Dang, F;Zhang, L;Baba, Y
通讯作者：
Baba, Y