Arabidopsis desaturase 2 gene is involved in the regulation of cadmium and lead resistance
Arabidopsis desaturase 2 gene is involved in the regulation of cadmium and lead resistance
复制标题
拟南芥去饱和酶2基因参与镉和铅抗性的调节
DOI：
10.1007/s11104-012-1186-7
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发表时间：
2012-03
期刊：
影响因子：
4.9
通讯作者：
曹树青
中科院分区：
文献类型：
--
作者：
曹树青
目的 前期分离拟南芥（Arabidopsis thaliana）去饱和酶2（ADS2）cDNA，表明ADS2的表达具有器官依赖性并受低温上调。然而，人们对ADS2基因在植物抗重金属中的作用知之甚少。在这项研究中，我们表明ADS2基因参与了镉（Cd）和铅（Pb）抗性的调节。方法对于重金属抗性测试，种子在补充有指定浓度的金属离子的1/2 MS培养基上发芽和生长。为了量化根长度，植物在平板中垂直生长。对于重金属处理，在 MS 培养基上生长的两周龄野生型幼苗用镉 (Cd) 或铅 (Pb) 处理 24 小时，然后取样进行金属含量测量和 qPCR 分析。结果ADS2 受到 Cd(II) 的强烈抑制，ads2-1 突变体植物表现出增强的 Cd(II) 抗性。在ads2-1植物中检测到的Cd含量低于接受Cd(II)处理的野生型植物，这与AtPDR8基因表达的激活有关，AtPDR8基因是一种从细胞质中排除Cd(II)和/或含Cd(II)有毒化合物的泵，表明ADS2介导的Cd(II)抗性是AtPDR8依赖性的。我们还发现 ads2-1 植物表现出增加的 Pb(II) 敏感性，并且 ADS2 受到过氧化氢 (H2O2) 的强烈抑制，但不受 Pb(II) 的抑制。 ads2-1突变体表现出对H2O2和百草枯介导的氧化应激的敏感性增加，并且当受到Pb(II)和H2O2处理时，在ads2-1植物的叶子中观察到比野生型植物更高水平的H2O2积累，表明ADS2通过受损的ROS清除间接介导Pb(II)抗性。结论ADS2基因至少部分地通过两种不同的方式介导Cd(II)和Pb(II)抗性机制，分别是 AtPDR8 依赖机制和 ROS 解毒系统介导机制。
AimsIt was shown previously that Arabidopsis (Arabidopsis thaliana) desaturase 2 (ADS2) cDNA was isolated and it was shown that the expression of ADS2 was organ-dependent and up-regulated by low temperature. However, little is known about the role of ADS2 gene in heavy metal resistance in plants. In this study, we showed that ADS2 gene is involved in the regulation of cadmium (Cd) and lead (Pb) resistance.MethodsFor heavy metal resistance tests, seeds were germinated and grown on 1/2 MS media supplemented with the indicated concentrations of metal ions. To quantify root length, plants were grown vertically in plates. For heavy metal treatments, two-week old wild-type seedlings grown on MS media were treated with cadmium (Cd) or lead (Pb) for 24 h, and then sampled for metal content measurement and qPCR analysis.ResultsADS2 was strongly repressed by Cd(II), and ads2-1 mutant plants showed increased Cd(II) resistance. A lower Cd content was detected in ads2-1 plants than in wild-type plants subjected to Cd(II) treatment, which was associated with activation in expression of AtPDR8 gene, a pump excluding Cd(II) and/or Cd(II)-containing toxic compounds from the cytoplasm, suggesting that ADS2-mediated Cd(II) resistance is AtPDR8 dependent. We also found that ads2-1 plants showed increased Pb(II) sensitivity, and ADS2 was strongly repressed by hydrogen peroxide (H2O2) but not by Pb(II). The ads2-1 mutant showed increased sensitivity to oxidative stresses mediated by H2O2 and paraquat, and higher levels of H2O2 accumulation were observed in leaves of ads2-1 plants than those of wild-type plants when subjected to Pb(II) and H2O2, indicating that ADS2 mediates Pb(II) resistance indirectly by impaired ROS scavenging.ConclusionsADS2 gene mediates Cd(II) and Pb(II) resistance, at least in part, through two distinct mechanisms, an AtPDR8-dependent mechanism and a ROS detoxification system-mediated mechanism, respectively.
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