Synthesis of a polydopamaine nanoparticle/bacterial cellulose composite for use as a biocompatible matrix for laccase immobilization
Synthesis of a polydopamaine nanoparticle/bacterial cellulose composite for use as a biocompatible matrix for laccase immobilization
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聚多巴胺纳米颗粒/细菌纤维素复合材料的合成，用作漆酶固定的生物相容性基质
DOI：
10.1007/s10570-019-02588-6
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发表时间：
2019-10
期刊：
影响因子：
5.7
通讯作者：
Hu Jinguang
中科院分区：
文献类型：
--
作者：
Zhai Rui;Chen Xiangxue;Jin Mingjie;Hu Jinguang
摘要细菌纤维素因其优异的力学性能、纳米多孔结构、高纯度和超亲水性而成为固定化酶的支架材料。然而，由于其缺乏反应活性基团，与生物分子的结合能力相对较低，限制了其应用。在这项研究中，我们已经合成了聚多巴胺纳米粒子/细菌纤维素杂化材料与层次多尺度架构的酶固定化，通过一个简单的原位自组装聚多巴胺纳米粒子在纤维素表面。以漆酶为固定化酶，考察了固定化酶的反应时间、酶浓度、温度和pH值对固定化酶性能的影响。这种杂化复合材料表现出优异的酶固定化效率（约165 mg漆酶/g复合材料），并且固定化漆酶在较宽的温度范围内也表现出更好的热稳定性和操作稳定性，与游离漆酶相比。在染料脱色试验中，固定化漆酶具有快速、高效的染料去除能力和良好的可循环性，显示了其在各种工业废水处理工艺中的潜在应用。
AbstractBacterial cellulose has attracted attention as a scaffolding material for enzyme immobilization because of its excellent mechanical properties, nanoporous structure, high purity, and super hydrophilicity. However, the lack of reactive group and the relatively low binding capacity toward biomolecules limit its application. In this study, we have synthesized a polydopamine nanoparticle/bacterial cellulose hybrid material with hierarchical multiscale architecture for enzyme immobilization, via a simple in situ self-assembly of polydopamine nanoparticles on cellulose surface. Commercial enzyme laccases were employed to evaluate the enzyme immobilizability of this hybrid material, and various key parameters of enzyme immobilization such as reaction time, enzyme concentration, temperature and pH were also systematically studied. This hybrid composite displayed excellent enzyme immobilization efficiency (~ 165 mg laccase per g composite), and the immobilized laccase also exhibited better thermostability and operational stability over a broad temperature range as compared with free laccase. By using the immobilized laccase, fast and efficient dye removal ability and good recyclability were achieved during the dye decolorization test, which demonstrated its potential application in various industrial relevant wastewater treatment processes.Graphic abstract
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影响因子：
6.7
作者：
Lu, Shixiang;Yu, Jianying;Boukherroub, Rabah
通讯作者：
Boukherroub, Rabah
影响因子：
4.7
作者：
Gholizadeh, Reza;Wang, Yujun
通讯作者：
Wang, Yujun
DOI：
10.3390/molecules22020221
发表时间：
2017-02-01
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Chen J;Leng J;Yang X;Liao L;Liu L;Xiao A
通讯作者：
Xiao A
影响因子：
3
作者：
Jinning Zhang;Mingyu Song;Xiaoyu Wang;Jieru Wu;Zhanping Yang;Jianhua Cao;Yun Chen;Q. Wei
通讯作者：
Jinning Zhang;Mingyu Song;Xiaoyu Wang;Jieru Wu;Zhanping Yang;Jianhua Cao;Yun Chen;Q. Wei
影响因子：
0.5
作者：
P. Suwannawong;S. Khammuang;R. Sarnthima
通讯作者：
P. Suwannawong;S. Khammuang;R. Sarnthima