Fine particulate matter (PM(2.5)) enhances allergic sensitization in BALB/c mice.
Fine particulate matter (PM(2.5)) enhances allergic sensitization in BALB/c mice.
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DOI：
10.1080/15287394.2016.1222920
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Pinkerton KE
中科院分区：
文献类型：
--
作者：
Castañeda AR;Bein KJ;Smiley-Jewell S;Pinkerton KE
环境颗粒物（PM）是空气污染的一种成分，可加重哮喘患者的气道炎症和高反应性。研究表明，PM具有增强过敏性炎症反应的澄清剂样性质;然而，PM增强过敏反应的机制仍有待确定。本研究的目的是评估暴露于从萨克拉门托，CA收集的细颗粒物如何塑造BALB/c小鼠的过敏性气道免疫应答，这些小鼠接受致敏和卵清蛋白（OVA）激发。用磷酸盐缓冲盐水（PBS/PBS; n = 6）、PM/PBS（n = 6）、OVA/OVA（n = 6）或OVA + PM/OVA（n = 6）致敏/攻击八周龄BALB/c雄性小鼠。分析肺组织、支气管肺泡灌洗液（BALF）和血浆的细胞炎症、细胞因子、免疫球蛋白E和血红素氧合酶-1（HO-1）表达。与0 VA/0 VA动物相比，0 VA + PM/0 VA组中的小鼠显示显著增加的气道炎症。与OVA/OVA组相比，在OVA + PM/OVA中BALF灌洗液中回收的总细胞、巨噬细胞和嗜酸性粒细胞显著升高。组织学分级表明，与OVA/OVA相比，OVA + PM/OVA处理诱导显著的炎症。与PBS/PBS对照组相比，OVA/OVA和OVA + PM/OVA组的IgE和TNFα水平均显著增加。与OVA/OVA相比，用OVA + PM/OVA处理的小鼠的肺中HO-1阳性肺泡巨噬细胞的数量显著升高。我们的研究结果表明，细PM通过增加氧化应激机制增强肺组织的过敏性炎症反应。
Ambient particulate matter (PM), a component of air pollution, exacerbates airway inflammation and hyper-reactivity in asthmatic patients. Studies showed that PM possesses adjuvant-like properties that enhance the allergic inflammatory response; however, the mechanism(s) by which PM enhances the allergic response remains to be determined. The aim of this study was to assess how exposure to fine PM collected from Sacramento, CA, shapes the allergic airway immune response in BALB/c mice undergoing sensitization and challenge with ovalbumin (OVA). Eight-week old BALB/c male mice were sensitized/challenged with phosphate buffered saline (PBS/PBS; n = 6), PM/PBS (n = 6), OVA/OVA (n = 6), or OVA + PM/OVA (n = 6). Lung tissue, bronchoalveolar lavage fluid (BALF) and plasma were analyzed for cellular inflammation, cytokines, immunoglobulin E and heme oxygenase-1 (HO-1) expression. Mice in the OVA + PM/OVA group displayed significantly increased airway inflammation compared to OVA/OVA animals. Total cells, macrophages, and eosinophils recovered in BALF lavage were significantly elevated in the OVA + PM/OVA compared to OVA/OVA group. Histopathological grading indicated that OVA + PM/OVA treatment induced significant inflammation compared to OVA/OVA. Both IgE and TNFα levels were significantly increased in OVA/OVA and OVA + PM/OVA groups compared to PBS/PBS control. The number of HO-1 positive alveolar macrophages was significantly elevated in lungs of mice treated with OVA + PM/OVA compared to OVA/OVA. Our findings suggest that fine PM enhances allergic inflammatory response in pulmonary tissue through mechanisms involving increased oxidative stress.
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影响因子：
10.4
作者：
Li N;Wang M;Bramble LA;Schmitz DA;Schauer JJ;Sioutas C;Harkema JR;Nel AE
通讯作者：
Nel AE
影响因子：
5
作者：
Bein, K. J.;Wexler, A. S.
通讯作者：
Wexler, A. S.
影响因子：
2.8
作者：
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通讯作者：
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影响因子：
10.4
作者：
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通讯作者：
Peters J
DOI：
10.1152/ajplung.00115.2010
发表时间：
2010-09-01
影响因子：
4.9
作者：
Li, Ning;Harkema, Jack R.;Nel, Andre E.
通讯作者：
Nel, Andre E.