Low Neutralizing Antibody Titers against the Mu Variant of SARS-CoV-2 in 31 BNT162b2 Vaccinated Individuals in Colombia.
Low Neutralizing Antibody Titers against the Mu Variant of SARS-CoV-2 in 31 BNT162b2 Vaccinated Individuals in Colombia.
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DOI：
10.3390/vaccines10020180
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发表时间：
2022-01-24
期刊：
影响因子：
7.8
通讯作者：
Mercado-Reyes M
中科院分区：
文献类型：
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作者：
Álvarez-Díaz DA;Muñoz AL;Tavera-Rodríguez P;Herrera-Sepúlveda MT;Ruiz-Moreno HA;Laiton-Donato K;Franco-Muñoz C;Pelaez-Carvajal D;Cuellar D;Muñoz-Suarez AM;Galindo M;Arias-Ramírez EJ;Mercado-Reyes M
针对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的全球监测项目显示，出现了刺突蛋白突变的变体。基因组和实验室监测对于确定这些变体是否更具感染性或对抗病毒治疗和疫苗诱导的抗体较不敏感非常重要。在2021年流行病高峰期间，哥伦比亚分离出三种最主要的SARS-CoV-2变体：Mu，一种感兴趣的变体; Gamma，一种令人担忧的变体; B.1.111，缺乏与更强毒力相关的遗传标记。通过将每种SARS-CoV-2分离株的120个平均组织培养感染剂量（TCID 50）与来自31名BNT 162 b2疫苗接种志愿者的血清的5个2倍系列稀释液孵育来进行中和试验。通过Reed-Muench法计算平均中和滴度（MN 50）。截至8月底，Mu占哥伦比亚2019年冠状病毒病（COVID-19）病例的49%，其次是Gamma的25%。相比之下，B.1.111变得几乎无法检测。中和抗体的评价表明，接种BNT 162 b2疫苗的患者在显著低于B.1.111和Gamma的浓度下产生抗Mu变体的中和抗体滴度。这项研究表明了继续监测新出现的变异的重要性，以及评估其他疫苗诱导的中和抗体应答的必要性。
Global surveillance programs for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are showing the emergence of variants with mutations in the spike protein. Genomic and laboratory surveillance are important to determine if these variants may be more infectious or less susceptible to antiviral treatments and vaccine-induced antibodies. Three of the most predominant SARS-CoV-2 variants in Colombia during the epidemiological peaks of 2021 were isolated: Mu, a variant of interest; Gamma, a variant of concern; B.1.111, which lacks genetic markers associated with greater virulence. Microneutralization assays were performed by incubating 120 mean tissue culture infectious doses (TCID50) of each SARS-CoV-2 isolate with five two-fold serial dilutions of sera from 31 BNT162b2-vaccinated volunteers. The mean neutralization titer (MN50) was calculated by the Reed–Muench method. At the end of August, Mu represented 49% of coronavirus disease 2019 (COVID-19) cases in Colombia, followed by 25% of Gamma. In contrast, B.1.111 became almost undetectable. The evaluation of neutralizing antibodies suggests that patients vaccinated with BNT162b2 generate neutralizing antibody titers against the Mu variant at significantly lower concentrations relative to B.1.111 and Gamma. This study shows the importance of continuing surveillance programs of emerging variants, as well as the need to evaluate the neutralizing antibody response induced by other vaccines.
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BNT162B2 mRNA COVID-19疫苗的安全性和功效。
DOI：
10.1056/nejmoa2034577
发表时间：
2020-12-31
期刊：
The New England journal of medicine
影响因子：
--
作者：
Polack FP;Thomas SJ;Kitchin N;Absalon J;Gurtman A;Lockhart S;Perez JL;Pérez Marc G;Moreira ED;Zerbini C;Bailey R;Swanson KA;Roychoudhury S;Koury K;Li P;Kalina WV;Cooper D;Frenck RW Jr;Hammitt LL;Türeci Ö;Nell H;Schaefer A;Ünal S;Tresnan DB;Mather S;Dormitzer PR;Şahin U;Jansen KU;Gruber WC;C4591001 Clinical Trial Group
通讯作者：
C4591001 Clinical Trial Group
DOI：
10.3390/v13091687
发表时间：
2021-08-25
期刊：
Viruses
影响因子：
--
作者：
Rotondo JC;Martini F;Maritati M;Mazziotta C;Di Mauro G;Lanzillotti C;Barp N;Gallerani A;Tognon M;Contini C
通讯作者：
Contini C
DOI：
10.1016/j.meegid.2021.105038
发表时间：
2021-11
期刊：
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
影响因子：
--
作者：
Laiton-Donato K;Franco-Muñoz C;Álvarez-Díaz DA;Ruiz-Moreno HA;Usme-Ciro JA;Prada DA;Reales-González J;Corchuelo S;Herrera-Sepúlveda MT;Naizaque J;Santamaría G;Rivera J;Rojas P;Ortiz JH;Cardona A;Malo D;Prieto-Alvarado F;Gómez FR;Wiesner M;Martínez MLO;Mercado-Reyes M
通讯作者：
Mercado-Reyes M
影响因子：
64.5
作者：
Hoffmann M;Arora P;Groß R;Seidel A;Hörnich BF;Hahn AS;Krüger N;Graichen L;Hofmann-Winkler H;Kempf A;Winkler MS;Schulz S;Jäck HM;Jahrsdörfer B;Schrezenmeier H;Müller M;Kleger A;Münch J;Pöhlmann S
通讯作者：
Pöhlmann S
影响因子：
3.9
作者：
Laiton-Donato K;Usme-Ciro JA;Franco-Muñoz C;Álvarez-Díaz DA;Ruiz-Moreno HA;Reales-González J;Prada DA;Corchuelo S;Herrera-Sepúlveda MT;Naizaque J;Santamaría G;Wiesner M;Walteros DM;Ospina Martínez ML;Mercado-Reyes M
通讯作者：
Mercado-Reyes M