ZmFAR1 and ZmABCG26 Regulated by microRNA Are Essential for Lipid Metabolism in Maize Anther.
ZmFAR1 and ZmABCG26 Regulated by microRNA Are Essential for Lipid Metabolism in Maize Anther.
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microRNA 调控的 ZmFAR1 和 ZmABCG26 对于玉米花药的脂质代谢至关重要
DOI：
10.3390/ijms22157916
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发表时间：
2021-07-24
影响因子：
5.6
通讯作者：
Wan X
中科院分区：
文献类型：
--
作者：
Jiang Y;Li Z;Liu X;Zhu T;Xie K;Hou Q;Yan T;Niu C;Zhang S;Yang M;Xie R;Wang J;Li J;An X;Wan X
脂类代谢基因的功能和调控是植物雄性生殖所必需的。然而，非编码RNA对脂代谢核雄性不育（GMS）基因的表达调控还很不清楚。本研究利用转录组分析技术，系统预测了34个玉米白色棕色复合体成员的microRNA调控子。结果表明，ZmABCG 26转录物被预测为zma-miR 164 h-5 p的靶向，并且基于转录组数据和qRT-PCR实验两者，它们的表达水平在玉米B73和Oh 43遗传背景中呈负相关。在ZmABCG 26和另一种脂质代谢基因ZmFAR 1上进行CRISPR/Cas9诱导的基因诱变。DNA测序、表型和细胞学观察表明，ZmABCG 26和ZmFAR 1都是玉米的核不育基因。值得注意的是，ZmABCG 26蛋白定位于内质网（ER）、叶绿体/质体和质膜中。ZmFAR 1在体外对三种CoA底物均表现出催化活性，活性顺序为C12：0-CoA > C16：0-CoA > C18：0-CoA，其4个关键氨基酸位点对其催化活性起关键作用。脂质组学分析表明，减少角质量和增加蜡含量在两个zmabcg 26和zmfar 1不育突变体的花药。一个更详细的分析表明，野生型和突变体之间，以及zmabcg 26和zmfar 1之间的54个单体含量的差异变化。这些发现将有助于深入了解玉米花药中miRNA调控的脂类代谢基因以及脂类代谢基因的功能多样性，从而促进玉米花药中脂类的生物合成。此外，还开发了ZmABCG 26和ZmFAR 1的共分离分子标记，为不育系的选育提供了便利。
The function and regulation of lipid metabolic genes are essential for plant male reproduction. However, expression regulation of lipid metabolic genic male sterility (GMS) genes by noncoding RNAs is largely unclear. Here, we systematically predicted the microRNA regulators of 34 maize white brown complex members in ATP-binding cassette transporter G subfamily (WBC/ABCG) genes using transcriptome analysis. Results indicate that the ZmABCG26 transcript was predicted to be targeted by zma-miR164h-5p, and their expression levels were negatively correlated in maize B73 and Oh43 genetic backgrounds based on both transcriptome data and qRT-PCR experiments. CRISPR/Cas9-induced gene mutagenesis was performed on ZmABCG26 and another lipid metabolic gene, ZmFAR1. DNA sequencing, phenotypic, and cytological observations demonstrated that both ZmABCG26 and ZmFAR1 are GMS genes in maize. Notably, ZmABCG26 proteins are localized in the endoplasmic reticulum (ER), chloroplast/plastid, and plasma membrane. Furthermore, ZmFAR1 shows catalytic activities to three CoA substrates in vitro with the activity order of C12:0-CoA > C16:0-CoA > C18:0-CoA, and its four key amino acid sites were critical to its catalytic activities. Lipidomics analysis revealed decreased cutin amounts and increased wax contents in anthers of both zmabcg26 and zmfar1 GMS mutants. A more detailed analysis exhibited differential changes in 54 monomer contents between wild type and mutants, as well as between zmabcg26 and zmfar1. These findings will promote a deeper understanding of miRNA-regulated lipid metabolic genes and the functional diversity of lipid metabolic genes, contributing to lipid biosynthesis in maize anthers. Additionally, cosegregating molecular markers for ZmABCG26 and ZmFAR1 were developed to facilitate the breeding of male sterile lines.
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影响因子：
14.9
作者：
Chen C;Ridzon DA;Broomer AJ;Zhou Z;Lee DH;Nguyen JT;Barbisin M;Xu NL;Mahuvakar VR;Andersen MR;Lao KQ;Livak KJ;Guegler KJ
通讯作者：
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影响因子：
14.9
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通讯作者：
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影响因子：
7.4
作者：
Chen, Xiaoyang;Zhang, Hua;Jin, Weiwei
通讯作者：
Jin, Weiwei
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DOI：
10.1073/pnas.2010255117
发表时间：
2020-09-22
影响因子：
11.1
作者：
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通讯作者：
Wan X