Expanding the boundaries of atomic spectroscopy at the single-cell level: critical review of SP-ICP-MS, LIBS and LA-ICP-MS advances for the elemental analysis of tissues and single cells.
Expanding the boundaries of atomic spectroscopy at the single-cell level: critical review of SP-ICP-MS, LIBS and LA-ICP-MS advances for the elemental analysis of tissues and single cells.
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DOI：
10.1007/s00216-023-04721-8
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发表时间：
2023-11
影响因子：
4.3
通讯作者：
Felipe-Sotelo, Monica
中科院分区：
文献类型：
--
作者：
Davison, Claire;Beste, Dany;Bailey, Melanie;Felipe-Sotelo, Monica
金属在微生物学中起着至关重要的作用，需要精确的方法对其进行鉴定和定量。无法评估细胞异质性被认为是成功治疗不同疾病的障碍。与批量方法不同，单细胞分析允许基因相同人群的元素异质性与特定生物事件和药物有效性相关。单粒子电感耦合等离子体质谱（SP-ICP-MS）可以分析悬浮液中的单个细胞并测量这种异质性。在这里，我们探讨仪器设计的进展，比较质量分析仪和讨论需要优化的关键参数。这篇综述已经确定，细胞悬浮液和细胞固定方法的预处理效果需要进一步研究，并且需要新的验证方法，因为使用批量测量是不令人满意的。SP-ICP-MS的优点是可以分析大量的细胞；然而，它不提供空间信息。基于激光烧蚀（LA）的技术能够在单细胞水平上进行元素映射，例如激光诱导击穿光谱（LIBS）和激光烧蚀-电感耦合等离子体质谱（LA- icp - ms）。商用LIBS仪器的灵敏度限制了其在亚组织应用中的使用；然而，分析内源性大块成分的能力与纳米libs技术的发展相结合，显示出细胞研究的巨大潜力。LA-ICP-MS为单细胞的直接分析提供了高灵敏度，但标准化需要进一步发展。这些微量元素分析技术的结合及其与单细胞分析的多组学技术的耦合在回答基本生物学问题方面具有巨大的潜力。
Metals have a fundamental role in microbiology, and accurate methods are needed for their identification and quantification. The inability to assess cellular heterogeneity is considered an impediment to the successful treatment of different diseases. Unlike bulk approaches, single-cell analysis allows elemental heterogeneity across genetically identical populations to be related to specific biological events and to the effectiveness of drugs. Single particle-inductively coupled plasma-mass spectrometry (SP-ICP-MS) can analyse single cells in suspension and measure this heterogeneity. Here we explore advances in instrumental design, compare mass analysers and discuss key parameters requiring optimisation. This review has identified that the effect of pre-treatment of cell suspensions and cell fixation approaches require further study and novel validation methods are needed as using bulk measurements is unsatisfactory. SP-ICP-MS has the advantage that a large number of cells can be analysed; however, it does not provide spatial information. Techniques based on laser ablation (LA) enable elemental mapping at the single-cell level, such as laser-induced breakdown spectroscopy (LIBS) and laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS). The sensitivity of commercial LIBS instruments restricts its use for sub-tissue applications; however, the capacity to analyse endogenous bulk components paired with developments in nano-LIBS technology shows great potential for cellular research. LA-ICP-MS offers high sensitivity for the direct analysis of single cells, but standardisation requires further development. The hyphenation of these trace elemental analysis techniques and their coupling with multi-omic technologies for single-cell analysis have enormous potential in answering fundamental biological questions.
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DOI：
10.1039/c6an00123h
发表时间：
2016-04-21
期刊：
The Analyst
影响因子：
--
作者：
Elci SG;Yan B;Kim ST;Saha K;Jiang Y;Klemmer GA;Moyano DF;Tonga GY;Rotello VM;Vachet RW
通讯作者：
Vachet RW
影响因子：
2.8
作者：
Beste, Dany J. V.
通讯作者：
Beste, Dany J. V.
影响因子：
3.4
作者：
Giesen, Charlotte;Waentig, Larissa;Jakubowski, Norbert
通讯作者：
Jakubowski, Norbert
影响因子：
3.4
作者：
Ahmed, Irfan;Yang, Jingwei;Lau, Condon
通讯作者：
Lau, Condon
影响因子：
6.2
作者：
de Vega, Raquel Gonzalez;Goyen, Samantha;Clases, David
通讯作者：
Clases, David