Molecular analysis of hormone-regulated petal regeneration in Petunia
Molecular analysis of hormone-regulated petal regeneration in Petunia
复制标题
矮牵牛激素调节花瓣再生的分子分析
DOI：
10.1007/s00299-008-0544-7
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发表时间：
2008-04
影响因子：
6.2
通讯作者：
张宪省
中科院分区：
文献类型：
--
作者：
张宪省
花瓣是高等植物重要的花器官。为研究矮牵牛花瓣发育的机理，建立了矮牵牛花瓣离体再生体系。在含有n6 -苄基腺嘌呤（6-BA）和吲哚-3-乙酸（IAA）的组合培养基上，外植体直接高频诱导花瓣。对花发育相关基因的表达分析表明，这些基因可分为三类。在花瓣再生过程中诱导了叶花变异（ALF）基因。而b类、e类基因和参与细胞分裂的基因组成性上调。相比之下，c类和d类基因在外植体和再生组织中不表达。此外，原位杂交分析表明，ALF和GREEN PETAL （GP）的表达均受空间调控。结果表明，在花瓣再生过程中，激素的存在对基因表达进行了差异调控，花瓣再生可能需要激素调控基因的表达。本研究为进一步了解花瓣再生的机理提供了初步资料。
The petal is an important floral organ of higher plants. To study the mechanism of petal development, the in vitro regeneration system of petals was established in Petunia. High-frequency induction of petals occurred directly from explants on the media containing the combination of N6-benzyladenine (6-BA) and indole-3-acetic acid (IAA). Expression analysis of genes involved in flower development indicated that these genes were classified into three types. ABERRANT LEAF AND FLOWER (ALF) gene was induced during petal regeneration. Whereas, B-class and E-class genes, and genes involved in cell division were constitutively upregulated. In contrast, C-class and D-class genes were not expressed in explants and regenerated tissues. Further, in situ hybridization analysis showed that both ALF and GREEN PETAL (GP) expression were spatially regulated. The results suggest that differential regulation of gene expression occurs in the presence of hormones during petal regeneration, and hormone-regulated gene expression might be required for petal regeneration. This study provides the preliminary information to understand the mechanism of petal regeneration.
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影响因子：
11.4
作者：
Lu Wen-liang;Zhuang Ying-jie;K. Enomoto;Y. Fukunaga
通讯作者：
Lu Wen-liang;Zhuang Ying-jie;K. Enomoto;Y. Fukunaga
影响因子：
4.6
作者：
E. Souer;A. V. D. Krol;Daisy Kloos;C. Spelt;M. Bliek;J. Mol;R. Koes
通讯作者：
E. Souer;A. V. D. Krol;Daisy Kloos;C. Spelt;M. Bliek;J. Mol;R. Koes
影响因子：
6.2
作者：
An, YR;Li, XG;Zhang, XS
通讯作者：
Zhang, XS
影响因子：
6.2
作者：
Guan, C. M.;Zhu, S. S.;Zhang, X. S.
通讯作者：
Zhang, X. S.
影响因子：
10.5
作者：
Stuurman, J;J채ggi, F;Kuhlemeier, C
通讯作者：
Kuhlemeier, C