Data File Standard for Flow Cytometry, version FCS 3.1.
Data File Standard for Flow Cytometry, version FCS 3.1.
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DOI：
10.1002/cyto.a.20825
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发表时间：
2010-01
期刊：
影响因子：
3.7
通讯作者：
Brinkman, Ryan R.
中科院分区：
文献类型：
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作者：
Spidlen, Josef;Moore, Wayne;Parks, David;Goldberg, Michael;Bray, Chris;Bierre, Pierre;Gorombey, Peter;Hyun, Bill;Hubbard, Mark;Lange, Simon;Lefebvre, Ray;Leif, Robert;Novo, David;Ostruszka, Leo;Treister, Adam;Wood, James;Murphy, Robert E.;Roederer, Mario;Sudar, Damir;Zigon, Robert;Brinkman, Ryan R.
流式细胞术数据文件标准提供了在包含实验数据的文件范围内完整描述流式细胞术数据集所需的规范。1984年，第一个流式细胞术数据文件标准格式被采纳为FCS 1.0。该标准于1990年修订为FCS 2.0，1997年再次修订为FCS 3.0。我们在此报告下一代流式细胞术标准数据文件格式。FCS 3.1是根据社区建议的改进进行的一个小修订。该标准的不变目标是提供一种统一的文件格式，允许由一种类型的采集硬件和软件创建的文件被任何其他类型的采集硬件和软件分析。FCS 3.1标准保留了基本的FCS文件结构和该标准以前版本的大多数功能。FCS 3.1中包含的更改解决了以前版本中的潜在模糊性，并提供了更可靠的标准。主要的变化包括简化对国际字符的支持和改进对存储补偿的支持。主要增加的内容是支持首选显示比例、捕获样品体积的标准化方式、有关数据文件原始性的信息，以及支持高通量、基于平板的实验中的平板和微孔识别。有关变更的完整列表，请参见本手稿补充材料中FCS 3.1规范的规范版本（或在www.example.com的当前标准部分）。
The flow cytometry data file standard provides the specifications needed to completely describe flow cytometry data sets within the confines of the file containing the experimental data. In 1984, the first Flow Cytometry Standard format for data files was adopted as FCS 1.0. This standard was modified in 1990 as FCS 2.0 and again in 1997 as FCS 3.0. We report here on the next generation Flow Cytometry Standard data file format. FCS 3.1 is a minor revision based on suggested improvements from the community. The unchanged goal of the Standard is to provide a uniform file format that allows files created by one type of acquisition hardware and software to be analyzed by any other type. The FCS 3.1 standard retains the basic FCS file structure and most features of previous versions of the standard. Changes included in FCS 3.1 address potential ambiguities in the previous versions and provide a more robust standard. The major changes include simplified support for international characters and improved support for storing compensation. The major additions are support for preferred display scale, a standardized way of capturing the sample volume, information about originality of the data file, and support for plate and well identification in high throughput, plate based experiments. Please see the normative version of the FCS 3.1 specification in supplementary material to this manuscript (or at http://www.isac-net.org/ in the Current standards section) for a complete list of changes.
影响因子：
3.7
作者：
Lee, Jamie A.;Spidlen, Josef;Boyce, Keith;Cai, Jennifer;Crosbie, Nicholas;Dalphin, Mark;Furlong, Jeff;Gasparetto, Maura.;Goldberg, Michael;Goralczyk, Elizabeth M.;Hyun, Bill;Jansen, Kirstin;Kollmann, Tobias;Kong, Megan;Leif, Robert;McWeeney, Shannon;Moloshok, Thomas D.;Moore, Wayne;Nolan, Garry;Nolan, John;Nikolich-Zugich, Janko;Parrish, David;Purcell, Barclay;Qian, Yu;Selvaraj, Biruntha;Smith, Clayton;Tchuvatkina, Olga;Wertheimer, Anne;WilkinSon, Peter;Wilson, Christopher;Wood, James;Zigon, Robert;Scheuermann, Richard H.;Brinkman, Ryan R.
通讯作者：
Brinkman, Ryan R.
影响因子：
3.7
作者：
Spidlen, Josef;Leif, Robert C.;Brinkman, Ryan R.
通讯作者：
Brinkman, Ryan R.
DOI：
10.1002/(sici)1097-0320(19970601)28:2
发表时间：
1997-06-01
期刊：
CYTOMETRY
影响因子：
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作者：
Seamer, LC;Bagwell, CB;Murphy, RF
通讯作者：
Murphy, RF