Modulation of Macrophage Polarization by Carbon Nanodots and Elucidation of Carbon Nanodot Uptake Routes in Macrophages.
Modulation of Macrophage Polarization by Carbon Nanodots and Elucidation of Carbon Nanodot Uptake Routes in Macrophages.
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DOI：
10.3390/nano11051116
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发表时间：
2021-04-26
期刊：
影响因子：
--
通讯作者：
Jia Z
中科院分区：
文献类型：
--
作者：
Dunphy A;Patel K;Belperain S;Pennington A;Chiu NHL;Yin Z;Zhu X;Priebe B;Tian S;Wei J;Yi X;Jia Z
动脉粥样硬化是全球普遍关注的问题，因为它是心血管疾病的主要原因，也是一个巨大的公共福利问题。巨噬细胞在疾病状态的发生中起着关键作用，是血管研究和治疗的热门靶点。碳纳米点（CNDs）是一种碳基纳米材料，近年来因其在生物医学领域的潜在应用而备受关注。这项研究是表征巨噬细胞和CNDs之间相互作用的第一次尝试。我们使用THP-1单核细胞衍生的巨噬细胞作为我们的靶细胞系，代表了人体内的原代巨噬细胞。我们的研究结果表明，CNDs在各种剂量下都是无毒的。12- o-十四烷醇-13-乙酸酯（TPA）和0.1 mg/mL CNDs共同作用使THP-1单核细胞分化为巨噬细胞。这种联合治疗显著增加了cd206和cd68（参与吞噬的关键受体）的表达，并增加了CCL2（一种单核细胞趋化剂和促炎细胞因子）的表达。与0.1 mg/mL CNDs共处理的THP-1单核细胞源性巨噬细胞的吞噬活性也显著增加。此外，本研究还研究了CNDs进入巨噬细胞的潜在途径。我们已经证明了用诺可达唑（微管干扰剂）、n -苯氰苯甲酸（氯通道阻滞剂）和氯化汞（水通道蛋白通道抑制剂）处理巨噬细胞对CNDs摄取的抑制作用。总的来说，本研究提供了CNDs引起巨噬细胞功能改变的证据，并指出了多种潜在的进入途径。
Atherosclerosis represents an ever-present global concern, as it is a leading cause of cardiovascular disease and an immense public welfare issue. Macrophages play a key role in the onset of the disease state and are popular targets in vascular research and therapeutic treatment. Carbon nanodots (CNDs) represent a type of carbon-based nanomaterial and have garnered attention in recent years for potential in biomedical applications. This investigation serves as a foremost attempt at characterizing the interplay between macrophages and CNDs. We have employed THP-1 monocyte-derived macrophages as our target cell line representing primary macrophages in the human body. Our results showcase that CNDs are non-toxic at a variety of doses. THP-1 monocytes were differentiated into macrophages by treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA) and co-treatment with 0.1 mg/mL CNDs. This co-treatment significantly increased the expression of CD 206 and CD 68 (key receptors involved in phagocytosis) and increased the expression of CCL2 (a monocyte chemoattractant and pro-inflammatory cytokine). The phagocytic activity of THP-1 monocyte-derived macrophages co-treated with 0.1 mg/mL CNDs also showed a significant increase. Furthermore, this study also examined potential entrance routes of CNDs into macrophages. We have demonstrated an inhibition in the uptake of CNDs in macrophages treated with nocodazole (microtubule disruptor), N-phenylanthranilic acid (chloride channel blocker), and mercury chloride (aquaporin channel inhibitor). Collectively, this research provides evidence that CNDs cause functional changes in macrophages and indicates a variety of potential entrance routes.
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影响因子：
3.7
作者：
Hara K;Shirasuna K;Usui F;Karasawa T;Mizushina Y;Kimura H;Kawashima A;Ohkuchi A;Matsuyama S;Kimura K;Takahashi M
通讯作者：
Takahashi M
影响因子：
6.2
作者：
Hu, Qin;Meng, Xiangpeng;Chan, Wan
通讯作者：
Chan, Wan
影响因子：
3.1
作者：
Martin, DK;Boneham, GC;Campbell, TJ
通讯作者：
Campbell, TJ
影响因子：
2.5
作者：
Ren, Shiyan;Fan, Xueqiang;Liu, Peng
通讯作者：
Liu, Peng
纳米颗粒与食用植物的相互作用及其在食物链中的可能影响。
影响因子：
6.1
作者：
Rico CM;Majumdar S;Duarte-Gardea M;Peralta-Videa JR;Gardea-Torresdey JL
通讯作者：
Gardea-Torresdey JL