Effect of MOG sensitization on somatosensory evoked potential in Lewis rats.
Effect of MOG sensitization on somatosensory evoked potential in Lewis rats.
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DOI：
10.1016/j.jns.2009.04.025
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发表时间：
2009-09-15
影响因子：
4.4
通讯作者：
Kerr, Douglas A.
中科院分区：
文献类型：
--
作者：
All, Angelo H.;Walczak, Piotr;Agrawal, Gracee;Gorelik, Michael;Lee, Christopher;Thakor, Nitish V.;Bulte, Jeff W. M.;Kerr, Douglas A.
关键词：
髓鞘少突胶质细胞糖蛋白(MOG)通常被用作免疫原来诱导针对内源性髓鞘的免疫反应，从而在啮齿动物中建立多发性硬化症模型。当MOG与完全弗氏佐剂(CFA)联合使用时，随后会发生多灶性、多时相性疾病；而当MOG与不完全弗氏佐剂(IFA)联合使用时，通常不会出现临床疾病。脊髓内注射细胞因子和溴化乙锭(EtBR)可诱导MOG-IFA免疫动物发生神经系统疾病。在这项研究中，我们调查了MOG-IFA免疫的大鼠是否出现了躯体感觉诱发电位(SEP)记录所定义的亚临床损伤。抗MOG-125抗体的滴定显示，MOG免疫的大鼠周围有针对髓鞘的强大免疫反应。然而，SEP指标并没有随着时间的推移而发生显着变化。MOG致敏后脊髓内注射细胞因子-EtBR后，SEP波幅降低，潜伏期延长，提示脊髓背柱轴突损伤和脱髓鞘。这些发现后来通过T2加权MRI和脊髓组织学苏木精-伊红染色得到证实。这份报告证实，MOG-IFA单独免疫不会改变SEP相关神经通路的神经元传导，体内纵向SSEP记录为大鼠局灶性脊髓炎(MOG-IFA和脊髓内细胞因子-EtBR)提供了一种灵敏的读数。
Myelin oligodendrocyte glycoprotein (MOG) is commonly used as an immunogen to induce an immune response against endogenous myelin, thereby modeling multiple sclerosis in rodents. When MOG is combined with complete Freund’s adjuvant (CFA), multifocal, multiphasic disease ensues; whereas when MOG is combined with incomplete Freund’s adjuvant (IFA), clinical disease is usually absent. MOG-IFA immunized animals can be induced to have neurological disease after intraspinal injections of cytokines and ethidium bromide (EtBr). In this study, we investigated whether MOG-IFA immunized rats exhibited subclinical injury as defined by Somatosensory Evoked Potential (SEP) recordings. The titration of Anti-MOG-125 antibodies showed robust peripheral mounting of immune response against myelin in MOG-immunized rats. However the SEP measures showed no significant change over time. Upon injecting cytokine-EtBr in the spinal cord after MOG sensitization, the SEP recordings showed reduced amplitude and prolonged latency, suggestive of axonal injury and demyelination in the dorsal column, respectively. These findings were later confirmed using T2-weighted MRI and histological hematoxilin-eosin stain of the spinal cord. This report establishes that MOG-IFA immunization alone does not alter neuronal conduction in SEP-related neural-pathways and that longitudinal in-vivo SSEP recordings provide a sensitive read-out for focal myelitis (MOG-IFA & intraspinal cytokine-EtBr) in rats.
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影响因子：
15.9
作者：
DEROSBO, NK;MILO, R;BENNUN, A
通讯作者：
BENNUN, A
影响因子：
3.3
作者：
SCOLDING, NJ;FRITH, S;COMPSTON, DAS
通讯作者：
COMPSTON, DAS
影响因子：
4.2
作者：
BASSO, DM;BEATTIE, MS;BRESNAHAN, JC
通讯作者：
BRESNAHAN, JC
影响因子：
2
作者：
Agrawal, Gracee;Thakor, Nitish V.;All, Angelo H.
通讯作者：
All, Angelo H.
DOI：
10.1016/s0013-4694(97)00117-x
发表时间：
1998-02-01
期刊：
ELECTROENCEPHALOGRAPHY AND CLINICAL NEUROPHYSIOLOGY
影响因子：
--
作者：
Nuwer, MR
通讯作者：
Nuwer, MR