Ontogeny of globin expression in zebrafish (Danio rerio)
Ontogeny of globin expression in zebrafish (Danio rerio)
复制标题
斑马鱼（斑马鱼）珠蛋白表达的个体发育
DOI：
10.1007/s00360-011-0588-9
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
T. Burmester
中科院分区：
文献类型：
--
作者：
J. Tiedke;Frank Gerlach;Stephanie A. Mitz;T. Hankeln;T. Burmester
呼吸蛋白负责运输和储存氧气。众所周知，脊椎动物个体发育过程中对氧的特定需求导致血红蛋白链表达的转换。在这里，我们的特点是斑马鱼（Danio rerio）球蛋白的发育概况通过实时定量逆转录PCR。估计了血红蛋白链（包括新鉴定的胚胎α链）以及肌红蛋白、神经红蛋白、细胞红蛋白1和2以及珠蛋白X的总mRNA水平。在未受精卵中检测到所有球蛋白的mRNA，表明母体储存。胚胎α-和β-血红蛋白mRNA在孵化时达到峰值，并在16 dpf时转换为成体血红蛋白表达。在受精后31 h左右，肌红蛋白mRNA水平增强，与心脏和肌肉的发育相一致，而脑红蛋白mRNA的表达模式与神经系统的形成有关。肌红蛋白和神经红蛋白mRNA的量在整个个体发育的一个数量级内是相似的，暂时支持神经红蛋白的呼吸作用。细胞珠蛋白2 mRNA水平逐渐增加，而细胞珠蛋白1 mRNA水平在~31 hpf后强烈增加，这与细胞增殖中的功能一致。珠蛋白X mRNA水平在卵母细胞中最高，但在后期较低。总之，这些数据表明了每种球蛋白的特定作用，这也与鱼类发育中的某些事件有关。
Respiratory proteins are responsible for transport and storage of oxygen. It is well established that specific requirements for oxygen during vertebrate ontogeny cause switches of hemoglobin chain expression. Here, we characterize the developmental profiles of zebrafish (Danio rerio) globins by means of quantitative real-time reverse transcription PCR. The total mRNA levels of the hemoglobin chains, including a newly identified embryonic α-chain, as well as myoglobin, neuroglobin, cytoglobin 1 and 2, and globin X were estimated. mRNAs of all globins were detectable in unfertilized eggs, suggesting maternal storage. Embryonic α- and β-hemoglobin mRNA peaked at hatching and the switch to adult hemoglobin expression occurred 16 dpf. Enhanced myoglobin mRNA levels were detected ~31 h post-fertilization (hpf), coinciding with the heart and the muscle development, while neuroglobin mRNA expression pattern correlates with the formation of the nervous system. Amounts of myoglobin and neuroglobin mRNA were similar within an order of magnitude throughout the ontogeny, tentatively supporting a respiratory role of neuroglobin. Cytoglobin 2 mRNA levels increased gradually, whereas cytoglobin 1 mRNA levels increased strongly after ~31 hpf, which is in agreement with a function in cell proliferation. Globin X mRNA level was highest in oocytes, but low in later stages. Together, these data suggest a specific role for each globin, which are also associated with certain events in fish development.
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影响因子：
2.7
作者：
Brownlie, A;Hersey, C;Zon, L
通讯作者：
Zon, L
影响因子：
4.6
作者：
D. Stainier;Robert K. K. Lee-Robert-K.-K.-Lee-2107716099;M. Fishman
通讯作者：
D. Stainier;Robert K. K. Lee-Robert-K.-K.-Lee-2107716099;M. Fishman
影响斑马鱼视网膜发育的突变。
DOI：
10.1242/dev.123.1.263
发表时间：
1996
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Malicki,J;Neuhauss,SC;Schier,AF;Solnica-Krezel,L;Stemple,DL;Stainier,DY;Abdelilah,S;Zwartkruis,F;Rangini,Z;Driever,W
通讯作者：
Driever,W
影响因子：
6.1
作者：
VANBLERKOM, J;DAVIS, PW;LEE, J
通讯作者：
LEE, J