Prevalence and characterization of quinolone resistance in Laribacter hongkongensis from grass carp and Chinese tiger frog.
Prevalence and characterization of quinolone resistance in Laribacter hongkongensis from grass carp and Chinese tiger frog.
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草鱼和中华虎蛙香港落叶松杆菌喹诺酮耐药性的流行和特征。
DOI：
10.1099/jmm.0.059451-0
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发表时间：
2013-10
影响因子：
3
通讯作者：
Wu, Ai-Wu
中科院分区：
文献类型：
--
作者：
Luo, Yu-Ting;Mao, Min-Jie;Lin, Yong-Ping;Wu, Ai-Wu
香港落叶松杆菌是一种食源性细菌，与社区获得性胃肠炎和腹泻有关。最近报道了水产品中分离的细菌存在喹诺酮类耐药性，但耐药性的分子机制仍不清楚。本研究从草鱼（n = 443）和中华虎蛙（n = 171）中分离出总共 157 个 L. hongkongensis 菌株。对 21 种环丙沙星耐药菌株的喹诺酮耐药决定区 (QRDR) 突变、获得性喹诺酮耐药 (AQR) 基因以及外排泵在耐药中的作用进行了分析。发现gyrA（密码子85和89）和parC（密码子83和231）中的所有QRDR突变与环丙沙星耐药性密切相关。 42.9%（9/21）的耐药菌株中发现AQR基因aac(6')-Ib-cr，但未检测到qnrA、qnrB、qnrC、qnrD、qnrS和qepA。在存在外排泵抑制剂的情况下，未观察到环丙沙星的 MIC 发生显着变化，表明外排泵的作用可能不存在。所有 21 种环丙沙星耐药菌株均显示出不同的电泳模式，这表明它们没有遗传相关性。这些数据强调了QRDR突变和AQR基因aac(6')-Ib-cr在香港乳杆菌异质群体中喹诺酮耐药性发展过程中的重要性。
Laribacter hongkongensis is a food-borne bacterium associated with community-acquired gastroenteritis and diarrhoea. Quinolone resistance was recently reported in bacterial isolates from aquatic products, but the molecular mechanisms for resistance were still unknown. In this study, a total of 157 L. hongkongensis strains were isolated from grass carps (n = 443) and Chinese tiger frogs (n = 171). Twenty-one ciprofloxacin-resistant strains were analysed for mutations in quinolone resistance-determining regions (QRDR), acquired quinolone resistance (AQR) genes and the role of efflux pumps in resistance. All QRDR mutations in gyrA (codons 85 and 89) and parC (codons 83 and 231) were found to be closely associated with ciprofloxacin resistance. The AQR gene aac(6')-Ib-cr was found in 42.9% (9/21) of the resistant strains, but qnrA, qnrB, qnrC, qnrD, qnrS and qepA were not detected. No significant change of MICs to ciprofloxacin was observed in the presence of an efflux pump inhibitor, indicating the role of efflux pump was probably absent. All 21 ciprofloxacin-resistant strains showed different electrophoretic patterns, which suggested they were not genetically related. These data highlight the importance of QRDR mutations and the AQR gene aac(6')-Ib-cr during the development of quinolone resistance in a heterogeneous population of L. hongkongensis.
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中国水产品中整合子相关抗生素耐药香港落叶杆菌的鉴定和表征
DOI：
10.1016/j.ijfoodmicro.2010.10.014
发表时间：
2011-01-05
影响因子：
5.4
作者：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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