In vivo performance of an acellular disc-like angle ply structure (DAPS) for total disc replacement in a small animal model.
In vivo performance of an acellular disc-like angle ply structure (DAPS) for total disc replacement in a small animal model.
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DOI：
10.1002/jor.23310
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发表时间：
2017-01
期刊：
影响因子：
--
通讯作者：
Mauck RL
中科院分区：
文献类型：
--
作者：
Martin JT;Kim DH;Milby AH;Pfeifer CG;Smith LJ;Elliott DM;Smith HE;Mauck RL
生物工程椎间盘全椎间盘置换术可能是治疗终末期椎间盘疾病的一种替代方法。在之前的工作中，我们开发了复制天然椎间盘结构和功能的盘状角层结构（DAPS），并建立了大鼠尾巴模型来评估DAPS在体内的作用。在这里，我们评估了一种策略，在体内植入后，内源性细胞可以定植在非细胞DAPS上，并形成由DAPS地形模板组织的细胞外基质。为此，将脱细胞DAPS植入大鼠的尾椎，并在12周内通过力学测试、组织学和显微计算机断层扫描进行评估。使用外固定装置稳定种植体部位，并采用不同的对照组来评估固定效果。植入后DAPS内组织形成牢固，植入物的压缩力学性能与原生运动节段相匹配。在椎间盘切除术或DAPS植入后，固定为纤维组织形成提供了稳定的位置，但最终导致骨融合，在长期植入后，节段出现椎间桥接，植入的DAPS加速了这一过程。因此，在DAPS植入后复制压缩力学性能的同时，必须开发积极防止融合的方法。未来的工作将集中于通过在融合一段时间后重新激活运动节段，传递促软骨形成因子，以及在植入前用细胞预先播种DAPS来限制融合。
Total intervertebral disc replacement with a biologic engineered disc may be an alternative to spinal fusion for treating end-stage disc disease. In previous work, we developed disc-like angle ply structures (DAPS) that replicate the structure and function of the native disc and a rat tail model to evaluate DAPS in vivo. Here, we evaluated a strategy in which, after in vivo implantation, endogenous cells could colonize the acellular DAPS and form an extracellular matrix organized by the DAPS topographical template. To do so, acellular DAPS were implanted into the caudal spines of rats and evaluated over 12 weeks by mechanical testing, histology, and microcomputed tomography. An external fixation device was used to stabilize the implant site and various control groups were included to evaluate the effect of immobilization. There was robust tissue formation within the DAPS after implantation and compressive mechanical properties of the implant matched that of the native motion segment. Immobilization provided a stable site for fibrous tissue formation after either a discectomy or a DAPS implantation, but bony fusion eventually resulted, with segments showing intervertebral bridging after long-term implantation, a process that was accelerated by the implanted DAPS. Thus, while compressive mechanical properties were replicated after DAPS implantation, methods to actively prevent fusion must be developed. Future work will focus on limiting fusion by remobilizing the motion segment after a period of integration, delivering pro-chondrogenic factors, and pre-seeding DAPS with cells prior to implantation.
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DOI：
10.1002/jor.22355
发表时间：
2013-08
期刊：
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
影响因子：
--
作者：
Martin JT;Gorth DJ;Beattie EE;Harfe BD;Smith LJ;Elliott DM
通讯作者：
Elliott DM
影响因子：
9.7
作者：
Martin, John T.;Milby, Andrew H.;Chiaro, Joseph A.;Kim, Dong Hwa;Hebela, Nader M.;Smith, Lachlan J.;Elliott, Dawn M.;Mauck, Robert L.
通讯作者：
Mauck, Robert L.
影响因子：
3
作者：
Kluba, T;Niemeyer, T;Gründer, T
通讯作者：
Gründer, T
影响因子：
3.3
作者：
Bertolo, Alessandro;Mehr, Marco;Stoyanov, Jivko V.
通讯作者：
Stoyanov, Jivko V.
DOI：
10.1073/pnas.1107094108
发表时间：
2011-08-09
影响因子：
11.1
作者：
Bowles, Robby D.;Gebhard, Harry H.;Bonassar, Lawrence J.
通讯作者：
Bonassar, Lawrence J.