Amphetamine-induced in vivo release of dopamine in the striatum is influenced by local pH.
Amphetamine-induced in vivo release of dopamine in the striatum is influenced by local pH.
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安非他明诱导体内纹状体中多巴胺的释放受到局部 pH 值的影响。
DOI：
10.1016/0024-3205(88)90332-3
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发表时间：
1988
期刊：
影响因子：
6.1
通讯作者：
McBride,WJ
中科院分区：
文献类型：
--
作者：
Guan,XM;McBride,WJ
使用两个双边放置的推拉插管在麻醉大鼠的纹状体中检查局部 pH 值对给予 d-安非他明 (AMPH) 后内源性多巴胺 (DA) 和 3, 4-二羟基苯乙酸 (DOPAC) 体内流出的影响。在 pH 7.3 和 6.4 下，DA 和 DOPAC 的基线流出值分别约为 0.2 和 25 pmoles/15 分钟。皮下注射 2 mg/kg AMPH 诱导 DA 释放在 pH 7.3 时增加 3 倍，在 pH 6.4 时诱导 DA 释放增加 21 倍。在这两种情况下，给药后约 30 分钟达到最大值。施用 AMPH 后，在两种 pH 条件下，DOPAC 的流出量均减少至相同程度（对照值的 20%）。体外数据表明，较低的 pH 值不会改变 DA 或 DOPAC 的回收率。此外，5 uM AMPH 局部灌注产生的 DA 释放在较低 pH 值（比基线增加 50 倍）下也比在生理 pH 值（比基线增加 10 倍）更大。然而，通过局部灌注 35 mM K+ 产生的刺激 DA 释放在两种 pH 值下是相同的。初步实验还表明，AMPH 诱导的血清素 (5-HT) 释放存在 pH 效应，但两种培养基中 5-HT 含量的差异并不像 DA 所获得的那样大。较低 pH 值下细胞外 DA 水平显着升高可能是由于 DA 摄取系统对 AMPH 的亲和力较高，从而对 DA 摄取产生更大的抑制并增强 DA 释放。数据还表明，在较低 pH 值下，AMPH 对 5-HT 吸收位点的亲和力增强。
The effect of local pH on the in vivo efflux of endogenous dopamine (DA) and 3, 4-dihydroxyphenylacetic acid (DOPAC) following administration of d-amphetamine (AMPH) was examined in the striatum of the anesthetized rat using two bilaterally placed push-pull cannulae. At both pH 7.3 and 6.4, the baseline efflux values for DA and DOPAC were approximately 0.2 and 25 pmoles/15 min, respectively. Subcutaneous injection of 2 mg/kg AMPH induced a 3-fold increase of DA release at pH 7.3 and a 21-fold increase of DA release at pH 6.4. In both cases, the maximum was reached at about 30 min after the drug administration. Following the administration of AMPH, the efflux of DOPAC was reduced to the same degree (20% of control values) under both pH conditions. In vitro data showed that the lower pH did not alter the recovery of DA or DOPAC. In addition, release of DA produced by local perfusion with 5 uM AMPH was also greater at the lower pH (50-fold increase over baseline) than at the physiological pH (10-fold increase over baseline). The stimulated DA release produced by local perfusion with 35 mM K+, however, was the same at both pH values. Preliminary experiments also indicated that there was a pH effect for AMPH-induced serotonin (5-HT) release but that the difference in the amount of 5-HT in the two media was not nearly as large as that obtained for DA. The markedly elevated level of extracellular DA at the lower pH might be due to a higher affinity of the DA uptake system for AMPH, thereby producing greater inhibition of DA uptake as well as enhanced DA release. The data also suggest an enhanced affinity of AMPH for 5-HT uptake sites at the lower pH.
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长期使用脑内透析体内测定 3,4-二羟基苯乙胺及其代谢物 3,4-二羟基苯乙酸
影响因子：
4.7
作者：
B. Westerink;M. Tuinte
通讯作者：
M. Tuinte
钾刺激和前体负荷对大鼠伏核体内多巴胺、血清素及其代谢物释放的影响。
影响因子：
6.1
作者：
Guan,XM;McBride,WJ
通讯作者：
McBride,WJ
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DOI：
--
发表时间：
1982
期刊：
Pharmacology, Biochemistry and Behavior
影响因子：
--
作者：
James M. Murphy;James M. Murphy;W. Mcbride;W. Mcbride;L. Lumeng;L. Lumeng;Ting;Ting
通讯作者：
Ting
大鼠脑体内未刺激和安非他明刺激的内源性去甲肾上腺素和多巴胺的释放
影响因子：
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作者：
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通讯作者：
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DOI：
--
发表时间：
1986
期刊：
Pharmacology, Biochemistry and Behavior
影响因子：
--
作者：
T. Sharp;T. Ljungberg;T. Zetterström;U. Ungerstedt
通讯作者：
U. Ungerstedt