Adjuvant effect of a novel water-soluble polysaccharide isolated from the stem of Physalis alkekengi L. var. francheti (Mast.) Makino
Adjuvant effect of a novel water-soluble polysaccharide isolated from the stem of Physalis alkekengi L. var. francheti (Mast.) Makino
复制标题
从 Physalis alkekengi L. var. 茎中分离的新型水溶性多糖的佐剂作用
DOI：
10.5897/jmpr.9000044
复制
发表时间：
2011-08
影响因子：
--
通讯作者：
Wang, Guiyun
中科院分区：
文献类型：
--
作者：
Shang, Dongjie;Zhang, Lili;Han, Shuying;Wang, Guiyun
从酸浆成熟茎中分离纯化出水溶性多糖(WSP)。加盟店(Mast.)Makino是一种传统的中草药。气相色谱分析表明，WSP是一种酸性杂多糖，由Rha、Ara、Gal、Glc和Gala组成，摩尔比为2.6：1.0：6.5：3.5：2.4。更重要的是，在本研究中，我们评估了WSP在ICR小鼠中的佐剂活性。用卵白蛋白(OVA)、OVA/WSP、OVA/Quila和生理盐水皮下免疫雄性ICR小鼠，间隔14天，共2次。最后一次免疫后两周处死小鼠，分析血清抗体效价。结果表明，WSP能显著提高血清中OVA特异性抗体(Ig G、Ig G1、Ig G2b)的滴度(P<0.0 5或P<0.0 1)。因此，WSP可以被认为是一种很有前途的佐剂，可以同时诱导Th1和Th2应答，以提高疫苗的效力。
--
关键词：中草药、酸浆、多糖、佐剂。
Water-soluble polysacchride (WSP) was isolated and purified from the mature stem ofPhysalis alkekengi L. var. francheti (Mast.) Makino, which is a traditional Chinese medicine herb. Gas chromatography (GC) analysis indicated that WSP was an acid heteropolysaccharide, which was composed of Rha, Ara, Gal, Glc and GalA in molar ratio of 2.6 : 1.0 : 6.5 : 3.5 : 2.4. More importantly, adjuvant activity of WSP was evaluated in ICR mice in present study. Male ICR mice were immunized subcutaneously with ovalbumin (OVA) alone, OVA/WSP, OVA/QuilA, and saline two times at 14-day interval. The mice were sacrificed two weeks after the last immunization to analyze antibody titers in serum. It was observed that WSP significantly enhanced OVA-specific antibody titers (IgG, IgG1, IgG2b) in serum when compared with OVA-injected mice (P<0.05 or P<0.01). Thus, WSP could be considered as a promising adjuvant eliciting both Th1 and Th2 responses to improve the efficacy of vaccine.
Key words: Herbal medicine, Physalis alkekengi L., polysaccharide, adjuvant.
登录
查看更多内容
从酸浆果实中分离的多糖介导的免疫增强作用的评价。
影响因子：
--
作者：
Li, Yonggang;Han, Shuying;Zhao, Hou;Liu, Yali;Fang, Jinbo;Wang, Guiyun
通讯作者：
Wang, Guiyun
影响因子：
3.8
作者：
Helvaci, S.;Kokdil, G.;Guvenc, A.
通讯作者：
Guvenc, A.
影响因子：
3.8
作者：
Keith Basey;J. Woolley
通讯作者：
Keith Basey;J. Woolley
DOI：
10.1016/0305-0491(96)00078-8
发表时间：
1996-10
期刊：
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
影响因子：
--
作者：
M. Vessal;M. Rasti;F. Kooshesh
通讯作者：
M. Vessal;M. Rasti;F. Kooshesh
影响因子：
5.5
作者：
Ragupathi G;Yeung KS;Leung PC;Lee M;Lau CB;Vickers A;Hood C;Deng G;Cheung NK;Cassileth B;Livingston P
通讯作者：
Livingston P