Evaluation of immunologic enhancement mediated by a polysaccharide isolated from the fruit of Physalis alkekengi L. var. francheti (Mast.) Makino
Evaluation of immunologic enhancement mediated by a polysaccharide isolated from the fruit of Physalis alkekengi L. var. francheti (Mast.) Makino
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从酸浆果实中分离的多糖介导的免疫增强作用的评价。
DOI：
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发表时间：
2011-03
影响因子：
--
通讯作者：
Wang, Guiyun
中科院分区：
文献类型：
--
作者：
Li, Yonggang;Han, Shuying;Zhao, Hou;Liu, Yali;Fang, Jinbo;Wang, Guiyun
研究酸浆成熟果实多糖（PPSB）的免疫增强作用。变种francheti（Mast.）牧野用单独的卵清蛋白（OVA）、OVA/PPSB、OVA/基拉和盐水以14天间隔皮下免疫雄性ICR小鼠两次。在最后一次免疫后两周处死小鼠以分析体液和细胞免疫。进一步检测迟发型超敏反应（DTH）。通过溶血活性和急性毒性试验评价了PPSB的毒性。结果表明，PPSB组小鼠血清中OVA特异性抗体（IgG、IgG 1、IgG 2b）滴度、刀豆球蛋白（ConA）、脂多糖（LPS）、OVA诱导的脾细胞增殖、脾CD 4+和CD 8 + T淋巴细胞比例、DTH反应均显著高于OVA组（P0.05或P0.01）。毒性试验表明PPSB无毒。因此，PPSB既介导体液免疫又介导细胞免疫，且无毒。因此，PPSB应被认为是一个有前途的佐剂引发的Th 1和Th 2反应，以提高疫苗的效力。
关键词：中药，多糖，体液反应，细胞反应，佐剂。
The study was to investigate the immunologic enhancement mediated by a polysaccharide (designated PPSB) purified from the mature fruits of Physalis alkekengi L. var. francheti (Mast.) Makino. Male ICR mice were immunized subcutaneously with ovalbumin (OVA) alone, OVA/PPSB, OVA/QuilA, and saline two times at 14-day interval. The mice were sacrificed two weeks after the last immunization to analyze humoral and cellular immunity. Delayed-type hypersensitivity (DTH) response was further detected. Moreover, toxicity of PPSB was evaluated by haemolytic activity and acute toxicity assay. The results showed that PPSB significantly enhanced OVA-specific antibody titers (IgG, IgG1, IgG2b) in serum, the concanavalin (ConA), lipopolysaccharide (LPS), OVA-induced splenocyte proliferation, proportions of CD4+ and CD8+ T lymphocytes in spleen, DTH response when compared with OVA-injected mice (P < 0.05 or P < 0.01). Toxicity assay indicated PPSB was non-toxic. Therefore, PPSB mediated both humoral immunity and cellular immunity, and it was non-toxic. Thus, PPSB should be considered as a promising adjuvant eliciting both Th1 and Th2 responses to improve the efficacy of vaccine.
Key words: Herbal medicine, polysaccharide, humoral response, cellular response, adjuvant.
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影响因子：
5.5
作者：
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通讯作者：
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