Transcriptome analysis of the sex pheromone gland of the noctuid moth Heliothis virescens.
Transcriptome analysis of the sex pheromone gland of the noctuid moth Heliothis virescens.
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DOI：
10.1186/1471-2164-11-29
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发表时间：
2010-01-14
期刊：
影响因子：
4.4
通讯作者：
Groot AT
中科院分区：
文献类型：
--
作者：
Vogel H;Heidel AJ;Heckel DG;Groot AT
一千多种蛾类的性信息素的化学成分已被确定，但迄今为止，只有少数基因编码的酶负责这些化合物的生物合成已被确定。为了了解飞蛾性交流的进化，有必要知道哪些基因参与了特定信息素成分的产生，以及是什么控制了信息素混合物中它们相对频率的变化。利用转录组学方法对重要农业害虫夜蛾Heliothis virescens的信息素腺进行了表征，以获得大量的一般序列信息，并确定了一系列参与信息素生物合成途径的候选基因。为了方便鉴定参与广泛过程的基因集并捕获稀有转录本，我们从一个标准化的Heliothis virescens信息素腺（PG） cDNA文库中开发了大多数ESTs。将这些与非标准化库相结合，总共产生了17,233个est，其中组装成2,082个contigs和6,228个单例。利用BLAST对NR和Swissprot数据库的搜索，我们能够识别出大量假定的独特基因元件（unigenes），并将其与之前对Heliothis virescens幼虫期的转录组学调查结果进行比较。氧化石墨烯生物过程功能群的单基因分布表明，PG与幼虫的转录组总体相似，但PG中特定途径的富集程度不同。此外，我们在信息素生物合成途径中鉴定了大量候选基因。这些数据构成了夜蛾科第一批大规模est项目之一，是研究夜蛾科害虫急需的资源。我们的分析显示了一个令人惊讶的复杂转录组，我们发现了大量潜在的信息素生物合成途径和免疫相关基因，这些基因可以应用于Heliothis virescens和其他夜蛾科的群体和系统研究。
The chemical components of sex pheromones have been determined for more than a thousand moth species, but so far only a handful of genes encoding enzymes responsible for the biosynthesis of these compounds have been identified. For understanding the evolution of moth sexual communication, it is essential to know which genes are involved in the production of specific pheromone components and what controls the variation in their relative frequencies in the pheromone blend. We used a transcriptomic approach to characterize the pheromone gland of the Noctuid moth Heliothis virescens, an important agricultural pest, in order to obtain substantial general sequence information and to identify a range of candidate genes involved in the pheromone biosynthetic pathway. To facilitate identifying sets of genes involved in a broad range of processes and to capture rare transcripts, we developed our majority of ESTs from a normalized cDNA library of Heliothis virescens pheromone glands (PG). Combining these with a non-normalized library yielded a total of 17,233 ESTs, which assembled into 2,082 contigs and 6,228 singletons. Using BLAST searches of the NR and Swissprot databases we were able to identify a large number of putative unique gene elements (unigenes), which we compared to those derived from previous transcriptomic surveys of the larval stage of Heliothis virescens. The distribution of unigenes among GO Biological Process functional groups shows an overall similarity between PG and larval transcriptomes, but with distinct enrichment of specific pathways in the PG. In addition, we identified a large number of candidate genes in the pheromone biosynthetic pathways. These data constitute one of the first large-scale EST-projects for Noctuidae, a much-needed resource for exploring these pest species. Our analysis shows a surprisingly complex transcriptome and we identified a large number of potential pheromone biosynthetic pathway and immune-related genes that can be applied to population and systematic studies of Heliothis virescens and other Noctuidae.
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影响因子：
--
作者：
Gohl, Thomas;Krieger, Juergen
通讯作者：
Krieger, Juergen
影响因子：
2.2
作者：
Foster, SP
通讯作者：
Foster, SP
DOI：
10.1017/cbo9780511542664.009
发表时间：
2004-01-01
期刊：
ADVANCES IN INSECT CHEMICAL ECOLOGY
影响因子：
--
作者：
Cardé, RT;Haynes, KF
通讯作者：
Haynes, KF
影响因子：
2.2
作者：
BURTON R L
通讯作者：
BURTON R L
影响因子：
3.8
作者：
Groot, Astrid T.;Estock, Marie L.;Gould, Fred
通讯作者：
Gould, Fred