Associations of Alcohol Consumption With Epigenome-Wide DNA Methylation and Epigenetic Age Acceleration: Individual-Level and Co-twin Comparison Analyses.
Associations of Alcohol Consumption With Epigenome-Wide DNA Methylation and Epigenetic Age Acceleration: Individual-Level and Co-twin Comparison Analyses.
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饮酒与表观基因组DNA甲基化和表观遗传年龄加速的关系：个体水平和双胞胎比较分析。
DOI：
10.1111/acer.14528
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发表时间：
2021-03
期刊：
影响因子：
--
通讯作者：
Ollikainen M
中科院分区：
文献类型：
--
作者：
Stephenson M;Bollepalli S;Cazaly E;Salvatore JE;Barr P;Rose RJ;Dick D;Kaprio J;Ollikainen M
DNA甲基化可能在从正常饮酒到有问题饮酒的过程中发挥作用，并成为与酒精滥用相关的不良健康结果的基础。在目前的研究中，我们使用三种方法研究了饮酒与DNA甲基化模式之间的关联：传统的表观全基因组关联研究（EWAS）;双胞胎比较设计，控制双胞胎共享的遗传和环境影响;以及年龄加速的回归，定义为实际年龄和DNA甲基化年龄之间的差异，对饮酒的影响。参与者来自芬兰双胞胎队列（FinnTwin 12/FinnTwin 16; N = 1004; 55%女性;平均年龄= 23岁）。受试者报告了过去一周饮用的酒精饮料数量，并使用Infinium HumanMethylation 450 BeadChip在全血中评估了表观基因组范围的DNA甲基化。在EWAS中，饮酒与24个CpG位点的甲基化显著相关。当评估双胞胎兄弟姐妹（185对同卵双胞胎）之间的饮酒差异是否可以预测DNA甲基化的差异时，双胞胎比较从EWAS中复制了4个CpG位点，并确定了23个额外的位点。然而，当我们检查双胞胎之间饮酒模式的定性差异时（重度饮酒者与轻度饮酒者/戒酒者或中度饮酒者与戒酒者; 44对），甲基化模式在双胞胎中没有显着差异。最后，报告饮酒量较高的人也表现出更大的年龄加速，尽管在控制了共同双胞胎的遗传和环境影响后，结果不再显着。我们的分析提供了深入了解表观遗传变异和青年饮酒水平之间的关联。
DNA methylation may play a role in progression from normative to problematic drinking and underlie adverse health outcomes associated with alcohol misuse. In the current study, we examined the association between alcohol consumption and DNA methylation patterns using three approaches: a conventional epigenome-wide association study (EWAS); a co-twin comparison design, which controls for genetic and environmental influences that twins share; and a regression of age acceleration, defined as a discrepancy between chronological age and DNA methylation age, on alcohol consumption. Participants came from the Finnish Twin Cohorts (FinnTwin12/FinnTwin16; N = 1004; 55% female; average age = 23 years). Individuals reported the number of alcoholic beverages consumed in the past week, and epigenome-wide DNA methylation was assessed in whole-blood using the Infinium HumanMethylation450 BeadChip. In the EWAS, alcohol consumption was significantly related to methylation at 24 CpG sites. When evaluating whether differences between twin siblings (185 monozygotic pairs) in alcohol consumption predicted differences in DNA methylation, co-twin comparisons replicated four CpG sites from the EWAS and identified 23 additional sites. However, when we examined qualitative differences in drinking patterns between twins (heavy drinker versus light drinker/abstainer or moderate drinker versus abstainer; 44 pairs), methylation patterns did not significantly differ within twin pairs. Finally, individuals who reported higher alcohol consumption also exhibited greater age acceleration, though results were no longer significant after controlling for genetic and environmental influences shared by co-twins. Our analyses offer insight into the associations between epigenetic variation and levels of alcohol consumption in young adulthood.
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影响因子：
0.9
作者：
Kaprio, Jaakko
通讯作者：
Kaprio, Jaakko
影响因子：
4.4
作者：
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作者：
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DOI：
10.1111/j.1530-0277.2012.01757.x
发表时间：
2012-09-01
影响因子：
3.2
作者：
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通讯作者：
Kechris, Katerina J.
影响因子：
5.4
作者：
Järvenpää, T;Rinne, JO;Kaprio, J
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