Mitochondrial-derived reactive oxygen species play a vital role in the salicylic acid signaling pathway in Arabidopsis thaliana.
Mitochondrial-derived reactive oxygen species play a vital role in the salicylic acid signaling pathway in Arabidopsis thaliana.
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DOI：
10.1371/journal.pone.0119853
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Xing D
中科院分区：
文献类型：
--
作者：
Nie S;Yue H;Zhou J;Xing D
植物线粒体是活性氧的主要来源，被认为在防御反应的协调中起着信号细胞器的作用。目前，线粒体产生并整合的信号仍然有限。在这里，荧光技术用于监测线粒体在体内的事件，以及天然防御信号化学物质水杨酸（SA）对线粒体信号传导的诱导。在SA作用下的分离线粒体中观察到呼吸抑制。分离线粒体细胞色素还原酶活性分析表明，SA可能通过抑制活性直接作用于呼吸链复合体III。随着这种改变，线粒体ROS （mtROS）的快速爆发受到刺激。sa诱导的mtROS引起叶片组织或原生质体中表达线粒体- gfp （43C5）的线粒体形态转变和膜电位去极化。然而，应用H2O2清除剂AsA可显著阻止这两种事件，表明这两种事件都可归因于ROS积累。同时，sa诱导的mtROS上调AOX1a转录物丰度，这种诱导与抗病性相关，而asa预处理阻断了这种作用。研究结果表明，线粒体在sa诱导的ROS生成信号通路中发挥了重要作用，这可能为sa介导的植物防御反应等生物学过程提供了新的认识。
Plant mitochondria constitute a major source of ROS and are proposed to act as signaling organelles in the orchestration of defense response. At present, the signals generated and then integrated by mitochondria are still limited. Here, fluorescence techniques were used to monitor the events of mitochondria in vivo, as well as the induction of mitochondrial signaling by a natural defensive signal chemical salicylic acid (SA). An inhibition of respiration was observed in isolated mitochondria subjected to SA. The cytochrome reductase activity analysis in isolated mitochondria demonstrated that SA might act directly on the complex III in the respiration chain by inhibiting the activity. With this alteration, a quick burst of mitochondrial ROS (mtROS) was stimulated. SA-induced mtROS caused mitochondrial morphology transition in leaf tissue or protoplasts expressing mitochondria-GFP (43C5) and depolarization of membrane potential. However, the application of AsA, an H2O2 scavenger, significantly prevented both events, indicating that both of them are attributable to ROS accumulation. In parallel, SA-induced mtROS up-regulated AOX1a transcript abundance and this induction was correlated with the disease resistance, whereas AsA-pretreatment interdicted this effect. It is concluded that mitochondria play an essential role in the signaling pathway of SA-induced ROS generation, which possibly provided new insight into the SA-mediated biological processes, including plant defense response.
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