Physical mapping of 45S rDNA loci in Lilium OT hybrids and interspecific hybrids with Lilium regale
Physical mapping of 45S rDNA loci in Lilium OT hybrids and interspecific hybrids with Lilium regale
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百合 OT 杂交种和百合种间杂交种 45S rDNA 位点的物理定位
DOI：
10.1016/j.scienta.2019.03.035
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发表时间：
2019-06
影响因子：
4.3
通讯作者：
He Hengbin
中科院分区：
文献类型：
--
作者：
Cao Qinzheng;Lian Yuqin;Wang Lianjuan;Zhang Qian;Zhao Yuqian;Jia Guixia;He Hengbin
以东方百合(Oriental × )品种与百合(L.为培育兼具园艺性状和抗逆性的百合品种提供了一条可行的途径。利用荧光原位杂交(FISH)技术对百合45S核糖体DNA(RDNA)基因座进行物理定位，有助于了解百合育种中的遗传关系和染色体遗传。本研究从‘Conca d’Or‘×L.regale和’Saltarello‘×L.regale组合中获得了杂种后代，证实了OT品种作为母本与L.‘Saltarello’×L.regales的胚胎率最高(8.96)，表明‘Saltarello’与L.大吃大喝。根据杂交和FISH结果，分析了不同品种间的亲缘关系。并对其进行了进一步的研究。‘Saltarello’具有12个45S rDNA信号位点，其中8个与OFL相似。Regale，位于第1、2、5号染色体上，证实了与l.最远的是有五个相似标志的‘Conca d’Or‘和有三个相似标志的’Manissa‘。‘Saltarello’×L.rDNA杂交种的45S rDNA基因座位于第1~6号染色体上，数目在7~13条之间。这些杂交种既有整倍体，也有非整倍体，说明异源三倍体百合可以产生具有整倍体和非整倍体染色体数目的配子。因此，从OT杂交种的种间杂交种中选育新品种是可能的。
Crossing betweenLiliumOT (Oriental × Trumpet) cultivars andL. regalecould provide an accessible way to develop lily cultivars with both horticultural traits and stress resistance. Physical mapping of 45S ribosomal DNA (rDNA) loci by fluorescence in situ hybridization (FISH) can help understand genetic relationships and chromosome inheritance in lily breeding. In this study, hybrid progeny were obtained from the combinations of ‘Conca d’Or’ ×L. regaleand ‘Saltarello’ ×L. regale, confirming the fertility of OT cultivars as female parents in crosses withL. regale.‘Saltarello’ ×L. regalehad the highest embryo rate (8.96), indicating cross-compatibility between ‘Saltarello’ andL. regale. Based on the results of crossing and FISH, the genetic relationship between OT cultivars andL. regalewas further investigated. ‘Saltarello’ possessed twelve 45S rDNA signal loci, of which eight were similar to those ofL. regale, on chromosomes 1, 2 and 5, confirming the closest genetic relationship withL. regale, ‘Conca d’Or’ with five similar sigals second and ‘Manissa’ with three similar sigals farthest. The 45S rDNA loci in hybrids of ‘Saltarello’ ×L. regalewere located on chromosomes 1 through 6 and varied in number from 7 to 13. These hybrids included both euploids and aneuploids, demonstrating that allotriploidLiliumOT hybrids can produce viable gametes with euploid and aneuploid chromosome numbers. Thus, it should be possible to select new cultivars from interspecific hybrids of OT hybrids ×L. regale.
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影响因子：
1.6
作者：
C. Takahashi;I. Leitch;A. Ryan;M. D. Bennett;P. Brandham
通讯作者：
C. Takahashi;I. Leitch;A. Ryan;M. D. Bennett;P. Brandham
影响因子：
3.1
作者：
Maria Puertas;S. Xie;N. Khan;M. Ramanna;L. Niu;A. Marasek-Ciołakowska;P. Arens;J. M. van Tuyl
通讯作者：
Maria Puertas;S. Xie;N. Khan;M. Ramanna;L. Niu;A. Marasek-Ciołakowska;P. Arens;J. M. van Tuyl
影响因子：
2.4
作者：
Marasek-Ciolakowska A;Nishikawa T;Shea DJ;Okazaki K
通讯作者：
Okazaki K
DOI：
--
发表时间：
2007
期刊：
--
影响因子：
--
作者：
K. Lim;R. Gonzalez;Shujun Zhou;M. Ramanna;J. M. Tuyl
通讯作者：
K. Lim;R. Gonzalez;Shujun Zhou;M. Ramanna;J. M. Tuyl
影响因子：
3.1
作者：
Ki-Byung Lim;Jannie Wennekes;J. Jong;Evert Jacobsen;Jaap M. van Tuyl
通讯作者：
Ki-Byung Lim;Jannie Wennekes;J. Jong;Evert Jacobsen;Jaap M. van Tuyl