Analysis of tissue specimens by matrix-assisted laser desorption/ionization imaging mass spectrometry in biological and clinical research.
Analysis of tissue specimens by matrix-assisted laser desorption/ionization imaging mass spectrometry in biological and clinical research.
复制标题
DOI：
10.1021/cr3004295
复制
发表时间：
2013-04-10
期刊：
影响因子：
62.1
通讯作者：
Caprioli, Richard M.
中科院分区：
文献类型：
--
作者：
Norris, Jeremy L.;Caprioli, Richard M.
人类善于通过处理视觉信息从复杂系统中辨别相关信息。同样，当科学家努力了解复杂生物系统的基本性质时，他们继续依赖图像形式的视觉信息来表征和分类自然现象。旨在生成生物标本图像的新技术在我们现代生物学理解的发展中发挥了关键作用。最早的技术例子之一是 17 世纪应用光学显微镜分析生物组织，最终发现细胞是生物学的关键组成部分。(1) 幸运的是，科学家现在可视化生物系统的方式已经显着成熟。目前，生物样本成像方法涵盖了非常广泛的技术，利用许多不同的可测量物理现象来生成图像，从而深入了解样本内的潜在生物学。在上个世纪中，包括显微镜、放射线照相、超声检查和磁共振成像在内的许多成像技术为生物过程的可视化和医学实践做出了巨大贡献。（2）每种成像方式都有独特的优点和缺点，使它们能够为研究和临床实践做出贡献。成像仍然面临挑战的一个关键方面是将分子特定信息有效整合为图像的一部分。许多常用的体内成像技术可以产生高质量的图像，但这些图像不能表达为单个分子图像。尽管免疫染色可用于定位生物样品中的特定分子，但该方法取决于分子替代标记的使用，例如抗体或其他专用试剂，并且通常在单个实验中对一个或最多仅几个感兴趣的分子进行。
Human beings are adept at discerning relevant information from complex systems by processing visual information. Similarly, as scientists labor to understand the fundamental nature of complex biological systems, they have continued to rely on visual information in the form of images to characterize and classify natural phenomena. New technologies designed to produce images of biological specimens have played a key role in the development of our modern understanding of biology. One of the earliest technological examples, application of light microscopy to analysis of biological tissue in the 17th century, ultimately led to the discovery of the cell as a key component of biology.(1) Fortunately, the ways in which scientists now visualize biological systems have significantly matured. Currently, the methods for imaging biological specimens encompass an extraordinarily large range of technologies, capitalizing on many different measurable physical phenomena to produce images that provide insight into the underlying biology within the specimen. During the previous century, many imaging technologies including microscopy, radiography, ultrasonography, and magnetic resonance imaging have contributed greatly to visualization of biological processes and the practice of medicine.(2)Each imaging modality has unique advantages and disadvantages that enable them to make contributions to research and clinical practice. One key aspect of imaging that remains a challenge is effective integration of molecularly specific information as part of the image. Many of the commonly used in vivo imaging technologies produce high-quality images, but these cannot be expressed as individual molecular images. Although immunostaining can be used to localize specific molecules within a biological sample, this method depends upon the use of a surrogate marker of the molecule such as an antibody or other specialized reagent and is usually performed on one or at most only a few molecules of interest in a single experiment.
登录
查看更多内容
影响因子：
30.3
作者：
Attia, Ahmed S.;Schroeder, Kaitlin A.;Skaar, Eric P.
通讯作者：
Skaar, Eric P.
影响因子：
7.4
作者：
Baluya, Dodge L.;Garrett, Timothy J.;Yost, Richard A.
通讯作者：
Yost, Richard A.
影响因子：
48
作者：
Andersson, Malin;Groseclose, M. Reid;Caprioli, Richard M.
通讯作者：
Caprioli, Richard M.
影响因子：
7.4
作者：
Agar, Nathalie Y. R.;Malcolm, James G.;Mohan, Vandana;Yang, Hong W.;Johnson, Mark D.;Tannenbaum, Allen;Agar, Jeffrey N.;Blacks, Peter M.
通讯作者：
Blacks, Peter M.
影响因子：
7.4
作者：
Angel, Peggi M.;Spraggins, Jeffrey M.;Baldwin, H. Scott;Caprioli, Richard
通讯作者：
Caprioli, Richard