Expression of monocyte chemotactic protein 3 following simulated birth trauma in a murine model of obesity.
Expression of monocyte chemotactic protein 3 following simulated birth trauma in a murine model of obesity.
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DOI：
10.1016/j.urology.2010.07.466
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发表时间：
2010-12
期刊：
影响因子：
2.1
通讯作者：
Hijaz AK
中科院分区：
文献类型：
--
作者：
Vricella GJ;Tao M;Altuntas CZ;Liu G;Kavran M;Daneshgari F;Hijaz AK
通过相对MCP - 3表达来测定肥胖对瘦素缺乏型肥胖小鼠模拟分娩创伤的影响。
共有25只野生型和25只肥胖的C57BL/6未生育雌性小鼠分别接受1小时的阴道扩张（VD）、假阴道扩张或无阴道扩张的麻醉。然后在阴道扩张后立即或24小时采集盆腔器官组织，并随后进行实时聚合酶链反应分析。
野生型小鼠尿道MCP - 3水平在0小时时较基线升高，在阴道扩张组和假阴道扩张组中24小时时均恢复到基线水平。在肥胖小鼠中，假阴道扩张后0小时时MCP - 3水平较对照组升高6倍（P < 0.05），24小时时恢复到基线水平。在接受阴道扩张后，0小时时MCP - 3水平升高到基线值的6倍（P = 0.002），24小时时MCP - 3水平持续升高到对照组水平的15倍（P = 0.0003）。
在任何尿道操作后，野生型和肥胖小鼠的尿道组织中MCP - 3均显著过度表达。在24小时时，肥胖小鼠中阴道扩张组和假阴道扩张组的MCP - 3表达模式出现差异。随着创伤程度加重，24小时时MCP - 3持续升高，这表明潜在的肥胖导致对组织损伤的反应发生改变，且与损伤程度相关。这种关联使得有必要进一步研究MCP - 3作为干细胞迁移趋化因子的作用，以及其对分娩创伤后后续组织修复机制的影响。
To determine the effect of obesity on simulated birth trauma in leptin-deficient obese mice as measured by relative MCP-3 expression. A total of 25 wild-type and 25 obese C57BL/6 virgin female mice underwent 1 hour of vaginal distension (VD), sham VD or anesthesia without VD. Pelvic organ tissues were then harvested either immediately or 24-hours post VD and subsequent real-time PCR analysis was performed. Urethral MCP-3 levels in wild-type mice were elevated from baseline at 0-hours with a return to baseline at 24-hours in both VD and sham VD groups. In obese mice, there was a 6-fold elevation in MCP-3 levels at 0-hours after sham VD versus control (P < 0.05), which then returned to baseline levels at 24-hours. After undergoing VD, MCP-3 levels increased to 6-fold baseline values (P = 0.002) at 0-hours, with continued elevation in MCP-3 levels to 15 times control levels (P = 0.0003) at 24-hours. MCP-3 is significantly over expressed in the urethral tissues of both wild-type and obese mice immediately after any urethral manipulation. At 24-hours, the MCP-3 expression patterns become divergent between VD and sham VD in obese mice. With a greater degree of trauma, MCP-3 continued to rise at 24-hours, suggesting that the underlying obesity resulted in alterations in response to tissue injury paralleling the degree of injury. Such associations warrant further investigation into the role of MCP-3 as a chemokine for stem cell migration with implications for subsequent tissue repair mechanisms after birth trauma.
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DOI：
10.1111/j.1471-0528.1999.tb08407.x
发表时间：
1999-08-01
期刊：
BRITISH JOURNAL OF OBSTETRICS AND GYNAECOLOGY
影响因子：
--
作者：
Hojberg, KE;Salvig, JD;Secher, NJ
通讯作者：
Secher, NJ
影响因子：
9.8
作者：
Danforth, KN;Townsend, MK;Grodstein, F
通讯作者：
Grodstein, F
影响因子：
3.5
作者：
Penn, MS;Zhang, M;Topol, EJ
通讯作者：
Topol, EJ
DOI：
10.1111/j.1600-0412.1997.tb07993.x
发表时间：
1997-04-01
影响因子：
4.3
作者：
Rasmussen, KL;Krue, S;Agger, AO
通讯作者：
Agger, AO
影响因子：
6.6
作者：
BURGIO, KL;MATTHEWS, KA;ENGEL, BT
通讯作者：
ENGEL, BT