The comparison of four mitochondrial genomes reveals cytoplasmic male sterility candidate genes in cotton.
The comparison of four mitochondrial genomes reveals cytoplasmic male sterility candidate genes in cotton.
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四个线粒体基因组的比较揭示了棉花细胞质雄性不育候选基因
DOI：
10.1186/s12864-018-5122-y
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发表时间：
2018-10-26
期刊：
影响因子：
4.4
通讯作者：
Hua J
中科院分区：
文献类型：
--
作者：
Li S;Chen Z;Zhao N;Wang Y;Nie H;Hua J
背景高等植物线粒体基因组在大小、结构和序列含量上存在显著差异，重复DNA序列的积累和活性证明了这一点。线粒体基因组与核基因组的不相容导致男性生殖器官功能障碍，导致细胞质雄性不育(CMS)。结果比较了陆地棉不育系CMS-2074A和CMS-2074S及其恢复系和保持系的线粒体基因组。首先，我们注意到这些品系的有丝分裂组结构和序列都是保守的。其次，我们发现2074A和2074S的有丝分裂基因组发生了大规模的替换和重排。实际上，在2074A和2074S中分别有5个和6个独特的嵌合开放阅读框(ORF)，它们是由独特的重复序列与邻近的功能基因重组而来的。第三，在不育系(2074A)和可育恢复系(2074A)中发现了4个差异转录的嵌合型ORF。此外，我们的观察表明，棉花中的CMS与重排的加速速度有关，新的表达产物来自重组ORF。
BackgroundThe mitochondrial genomes of higher plants vary remarkably in size, structure and sequence content, as demonstrated by the accumulation and activity of repetitive DNA sequences. Incompatibility between mitochondrial genome and nuclear genome leads to non-functional male reproductive organs and results in cytoplasmic male sterility (CMS). CMS has been used to produce F1hybrid seeds in a variety of plant species.ResultsHere we compared the mitochondrial genomes (mitogenomes) ofGossypium hirsutumsterile male lines CMS-2074A and CMS-2074S, as well as their restorer and maintainer lines. First, we noticed the mitogenome organization and sequences were conserved in these lines. Second, we discovered the mitogenomes of 2074A and 2074S underwent large-scale substitutions and rearrangements. Actually, there were five and six unique chimeric open reading frames (ORFs) in 2074A and 2074S, respectively, which were derived from the recombination between unique repetitive sequences and nearby functional genes. Third, we found out four chimeric ORFs that were differentially transcribed in sterile line (2074A) and fertile-restored line.ConclusionsThese four novel and recombinant ORFs are potential candidates that confer CMS character in 2074A. In addition, our observations suggest that CMS in cotton is associated with the accelerated rates of rearrangement, and that novel expression products are derived from recombinant ORFs.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
4.4
作者：
Islam MS;Studer B;Byrne SL;Farrell JD;Panitz F;Bendixen C;Møller IM;Asp T
通讯作者：
Asp T
影响因子：
11.6
作者：
Alverson, Andrew J.;Rice, Danny W.;Palmer, Jeffrey D.
通讯作者：
Palmer, Jeffrey D.
影响因子：
10.7
作者：
Goremykin, Vadim V.;Salamini, Francesco;Viola, Roberto
通讯作者：
Viola, Roberto
影响因子：
5.1
作者：
Iwabuchi, M;Koizuka, N;Imamura, J
通讯作者：
Imamura, J