The genome and transcriptome of perennial ryegrass mitochondria.
The genome and transcriptome of perennial ryegrass mitochondria.
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DOI：
10.1186/1471-2164-14-202
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发表时间：
2013-03-23
期刊：
影响因子：
4.4
通讯作者：
Asp T
中科院分区：
文献类型：
--
作者：
Islam MS;Studer B;Byrne SL;Farrell JD;Panitz F;Bendixen C;Møller IM;Asp T
多年生黑麦草（Lolium perenne L.）是世界温带地区最重要的牧草和草坪草之一。它的线粒体基因组是母系遗传的，含有可以影响农业重要性状的基因。此外，线粒体基因组的DNA序列已经建立，并比较了大量的物种，以表征进化关系。因此，了解线粒体基因组的组织以及它在物种之间和物种内的变化是至关重要的。在这里，我们报告的第一个从头组装和注释的完整的线粒体基因组从多年生黑麦草。从多年生黑麦草叶片中分离完整的线粒体并用于mtDNA的提取。使用Roche 454 GS-FLX Titanium平台对线粒体基因组进行测序，覆盖率为167倍，并组装成678，580 bp的环状主分子。线粒体基因组共编码34种蛋白质、14种tRNA和3种rRNA，总基因空间为48，723 bp（7.2%）。此外，我们还发现了149个大于300 bp的开放阅读框，覆盖67，410 bp（9.93%）、250个SSR、29个串联重复序列、5对大重复序列和96对短反向重复序列。编码呼吸复合物亚基的基因-nad 1至nad 9、cob、cox 1至cox 3和atp 1至atp 9-在绝对数和归一化后均显示出高表达水平。这里提出的多年生黑麦草的线粒体基因组的环状主分子构成了一个重要的工具，为未来的尝试比较线粒体基因组内和草种之间。我们的研究结果还表明，多年生黑麦草的线粒体包含的基因是非常保守的单子叶植物物种的线粒体基因组中的能量生产的关键。表达分析使我们首次了解这些线粒体基因在多年生黑麦草中的转录组。
Perennial ryegrass (Lolium perenne L.) is one of the most important forage and turf grass species of temperate regions worldwide. Its mitochondrial genome is inherited maternally and contains genes that can influence traits of agricultural importance. Moreover, the DNA sequence of mitochondrial genomes has been established and compared for a large number of species in order to characterize evolutionary relationships. Therefore, it is crucial to understand the organization of the mitochondrial genome and how it varies between and within species. Here, we report the first de novo assembly and annotation of the complete mitochondrial genome from perennial ryegrass. Intact mitochondria from perennial ryegrass leaves were isolated and used for mtDNA extraction. The mitochondrial genome was sequenced to a 167-fold coverage using the Roche 454 GS-FLX Titanium platform, and assembled into a circular master molecule of 678,580 bp. A total of 34 proteins, 14 tRNAs and 3 rRNAs are encoded by the mitochondrial genome, giving a total gene space of 48,723 bp (7.2%). Moreover, we identified 149 open reading frames larger than 300 bp and covering 67,410 bp (9.93%), 250 SSRs, 29 tandem repeats, 5 pairs of large repeats, and 96 pairs of short inverted repeats. The genes encoding subunits of the respiratory complexes – nad1 to nad9, cob, cox1 to cox3 and atp1 to atp9 – all showed high expression levels both in absolute numbers and after normalization. The circular master molecule of the mitochondrial genome from perennial ryegrass presented here constitutes an important tool for future attempts to compare mitochondrial genomes within and between grass species. Our results also demonstrate that mitochondria of perennial ryegrass contain genes crucial for energy production that are well conserved in the mitochondrial genome of monocotyledonous species. The expression analysis gave us first insights into the transcriptome of these mitochondrial genes in perennial ryegrass.
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影响因子：
1.7
作者：
Jurka, J;Kapitonov, VV;Walichiewicz, J
通讯作者：
Walichiewicz, J
DOI：
10.1073/pnas.90.13.5934
发表时间：
1993-07-01
影响因子：
11.1
作者：
DEPAEPE, R;FORCHIONI, A;VEDEL, F
通讯作者：
VEDEL, F
DOI：
10.1073/pnas.042694899
发表时间：
2002-07-23
影响因子：
11.1
作者：
Adams, KL;Qiu, YL;Palmer, JD
通讯作者：
Palmer, JD
DOI：
10.1111/j.1471-8286.2005.01043.x
发表时间：
2005-12-01
期刊：
MOLECULAR ECOLOGY NOTES
影响因子：
--
作者：
Jensen, LB;Muylle, H;Barre, P
通讯作者：
Barre, P
影响因子：
16
作者：
Heinz S;Benner C;Spann N;Bertolino E;Lin YC;Laslo P;Cheng JX;Murre C;Singh H;Glass CK
通讯作者：
Glass CK