Fabrication of tissue engineering scaffolds through solid-state foaming of immiscible polymer blends.
Fabrication of tissue engineering scaffolds through solid-state foaming of immiscible polymer blends.
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DOI：
10.1088/1758-5082/3/4/045003
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发表时间：
2011-12
期刊：
影响因子：
9
通讯作者：
Yao D
中科院分区：
文献类型：
--
作者：
Zhou C;Ma L;Li W;Yao D
在基于支架的组织工程中，制造过程对于产生合适的微结构以供接种细胞生长和重组是重要的。在本文中，我们提出了一种新的方法，结合固态发泡和不混溶聚合物共混方法的支架制造。所提出的方法具有通用性的优点，并且能够产生宽范围的孔径和孔隙率。所提出的方法进行了研究与聚乳酸（PLA）和聚苯乙烯（PS）的共混物。通过首先使PLA/PS共混物发泡，然后提取PS相来产生互连的多孔结构。固态发泡实验在各种条件下进行，以实现所需的孔径。结果表明，适当的气体发泡参数可以使PLA/PS共混物中的PS相在发泡样品中更快地被提取出来，并且可以容易地控制支架的孔径。在发泡过程中获得的平均孔径范围为20-70 μm。PS萃取后，孔径和孔隙率都可以进一步提高。例如，在PS提取过程之后，孔径和孔隙率分别从48 μm和49%增加到59 μm和67%。制备的多孔支架用于培养人成骨细胞。细胞生长良好，逐渐形成纤维状结构。固相发泡与不相容聚合物共混相结合的方法为组织工程支架的制备提供了一种新的技术。
In scaffold-based tissue engineering, the fabrication process is important for producing suitable microstructures for seeded cells to grow and reformulate. In this paper, we present a new approach to scaffold fabrication by combining the solid-state foaming and the immiscible polymer blending method. The proposed approach has the advantage of being versatile and able to create a wide range of pore size and porosity. The proposed method is studied with polylactic acid (PLA) and polystyrene (PS) blends. The interconnected porous structure was created by first foaming the PLA/PS blend and then extracting the PS phase. The solid-state foaming experiments were conducted under various conditions to achieve the desired pore sizes. It is shown that the PS phase of the PLA/PS blend can be extracted much faster in the foamed samples and the pore size of the scaffolds can be easily controlled with proper gas foaming parameters. The average pore size achieved in the foaming process ranged from 20-70 μm. After PS extraction, both pore size and porosity can be further improved. For example, the pore size and porosity increased from 48 μm and 49% to 59 μm and 67%, respectively, after the PS extraction process. The fabricated porous scaffolds were used to culture human osteoblast cells. Cells grew well and gradually formed a fibrous structure. The combined solid-state foaming and immiscible polymer blending method provides a new technique for fabricating tissue engineering scaffolds.
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影响因子：
3.9
作者：
Das, S;Hollister, SJ;Widjaja, E
通讯作者：
Widjaja, E
DOI：
10.1002/jbm.820270207
发表时间：
1993-02-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
MIKOS, AG;BAO, Y;LANGER, R
通讯作者：
LANGER, R
影响因子：
14
作者：
Nam, YS;Park, TG
通讯作者：
Park, TG
影响因子：
56.9
作者：
LANGER, R;VACANTI, JP
通讯作者：
VACANTI, JP
影响因子：
14
作者：
MIKOS, AG;SARAKINOS, G;LANGER, R
通讯作者：
LANGER, R