Enhancement of oxidative stress contributes to increased pathogenicity of the invasive pine wood nematode
Enhancement of oxidative stress contributes to increased pathogenicity of the invasive pine wood nematode
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氧化应激的增强导致入侵松材线虫的致病性增加
DOI：
10.1098/rstb.2018.0323
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发表时间：
2019-01
影响因子：
6.3
通讯作者：
Sun Jianghua
中科院分区：
文献类型：
--
作者：
Zhang Wei;Zhao Lilin;Zhou Jiao;Yu Haiying;Zhang Chi;Lv Yunxue;Lin Zhe;Hu Songnian;Zou Zhen;Sun Jianghua
活性氧（ROS）在宿主植物对抗病原体的防御反应中发挥着重要作用。虽然 ROS 的产生和解毒已被充分了解，但不同病原体分离株克服宿主 ROS 的能力有何不同，或 ROS 对特定分离株致病性的贡献如何，在很大程度上仍有待探索。在这里，我们报道了 ROS 通路的转录调控与胰岛素通路相结合，增加了入侵物种松材线虫的致病性。结果表明，不同线虫分离株的繁殖力和致病力之间呈正相关。来自日本、中国和欧洲引入种群的强毒分离株的繁殖力显着高于来自美国的本地无毒分离株。入侵过程中 Mn-SOD 表达增加、过氧化氢酶/GPX-5 表达减少以及 H2O2 积累与强毒株相关。额外的H2O2可以提高Bu的繁殖力。松材线虫。此外，Mn-SOD 的消耗降低了 Bu 的繁殖力和毒力。松材线虫，而胰岛素途径则受到显着影响。因此，我们提出Bu具有破坏性致病性。松材线虫对松树的影响部分是由于与 ROS 途径相关的胰岛素途径调节的繁殖力上调，并通过 H2O2 氧化应激进一步增强。这些发现有助于更好地理解植物与病原体相互作用的致病机制和入侵物种的适应性进化。本文是主题“生物信号揭示智能害虫管理”的一部分。
Reactive oxygen species (ROS) play important roles in defence response of host plants versus pathogens. While generation and detoxification of ROS is well understood, how varied ability of different isolates of pathogens to overcome host ROS, or ROS contribution to a particular isolate's pathogenicity, remains largely unexplored. Here, we report that transcriptional regulation of the ROS pathway, in combination with the insulin pathway, increases the pathogenicity of invasive species Bursaphelenchus xylophilus. The results showed a positive correlation between fecundity and pathogenicity of different nematode isolates. The virulent isolates from introduced populations in Japan, China and Europe had significantly higher fecundity than native avirulent isolates from the USA. Increased expression of Mn-SOD and reduced expression of catalase/GPX-5 and H2O2 accumulation during invasion are associated with virulent strains. Additional H2O2 could improve fecundity of Bu. xylophilus. Furthermore, depletion of Mn-SOD decreased fecundity and virulence of Bu. xylophilus, while the insulin pathway is significantly affected. Thus, we propose that destructive pathogenicity of Bu. xylophilus to pines is partly owing to upregulated fecundity modulated by the insulin pathway in association with the ROS pathway and further enhanced by H2O2 oxidative stress. These findings provide a better understanding of pathogenic mechanisms in plant–pathogen interactions and adaptive evolution of invasive species. This article is part of the theme issue ‘Biotic signalling sheds light on smart pest management’.
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影响因子：
10.9
作者：
C. Yi;B. Byun;J. D. Park;S. I. Yang;K. Chang
通讯作者：
C. Yi;B. Byun;J. D. Park;S. I. Yang;K. Chang
DOI：
10.1163/187529279x00091
发表时间：
1979-01-01
期刊：
NEMATOLOGICA
影响因子：
--
作者：
MAMIYA, Y;ENDA, N
通讯作者：
ENDA, N
影响因子：
14.8
作者：
Ainsworth, Elizabeth A.;Gillespie, Kelly M.
通讯作者：
Gillespie, Kelly M.
棉铃虫真菌和细菌感染过程中棉铃虫免疫转录组的高通量分析
影响因子：
4.4
作者：
Xiong GH;Xing LS;Lin Z;Saha TT;Wang C;Jiang H;Zou Z
通讯作者：
Zou Z
影响因子：
4.4
作者：
Santos CS;Pinheiro M;Silva AI;Egas C;Vasconcelos MW
通讯作者：
Vasconcelos MW