Sorghum Bicolor L. leaf sheath polysaccharides: Dual frequency ultrasound-assisted extraction and desalination
Sorghum Bicolor L. leaf sheath polysaccharides: Dual frequency ultrasound-assisted extraction and desalination
复制标题
高粱叶鞘多糖：双频超声辅助提取和脱盐
DOI：
10.1016/j.indcrop.2018.10.032
复制
发表时间：
2018-12
影响因子：
5.9
通讯作者：
Yang Hongpeng
中科院分区：
文献类型：
--
作者：
Yarley Otu Phyllis Naa;Jiang Haonan;Zhou Cunshan;Yang Hongpeng
单频超声技术应用于超分子提取存在定向效应的缺点。因此，为了更好的产率而对材料进行的最佳破坏被削减。一个可能的解决方案是应用双频超声波。采用双频超声辅助提取（DF-UAE）高粱粗多糖（SL-C80 P）和离子液体双水相系统（ILATPS）纯化多糖（SL-D10 P），研究了不同超声频率对高粱多糖提取率的影响。采用响应面法（RSM）对叶鞘进行了优化。粗多糖和纯化多糖的实验收率分别为14.10%和97.17%，与预测值（14.88%和97.40%）非常接近。DF-UA透析后还观察到较高的（SL-D10 P）脱盐水平。两种样品的扫描电子显微镜下的表面形态和单糖组成的摩尔比不同，但傅里叶变换红外光谱相似。粗多糖和纯化的多糖具有螺旋结构，如刚果红测定所证明的，并分别记录分子量（1，202，000和12，950 g/mol）。在流变学方面，样品（SL-D10 P）显示出更大的弹性，而样品（SL-C80 P）显示出更大的粘度。最后，（SL-D10 P）在水中聚集，可被热分解，并具有显著的ABTS清除能力，因此具有显著的生物分子保护潜力。结果表明，该工艺提取的多糖得率较高。
Supramolecules extraction using single frequency ultrasound technology application has directional effect as a shortcoming. Thus, optimum disruption of materials for better yield is curtailed. One possible solution to this underperformance is the application of dual frequency ultrasound. Dual frequency ultrasound-assisted extraction (DF-UAE) of crude polysaccharide (SL-C80P) and ionic liquid aqueous two phase system (ILATPS) purified polysaccharide (SL-D10P) of sorghum (Sorghum bicolorL.) leaf sheath was achieved using response surface methodology (RSM). Experimental yield for crude and purified polysaccharide (14.10% and 97.17%) respectively was found to be very close to the predicted (14.88% and 97.40%). Higher desalination levels of (SL-D10P) was also observed after DF-UA dialysis. Surface morphology under scanning electron microscopy and molar ratios of monosaccharides composition of the two samples were different, but Fourier transform infrared spectroscopy was similar. Crude and purified polysaccharides were of helical structures as demonstrated by the congo red assay and recorded molecular weights (1,202,000 and 12,950 g/mol) respectively. The sample, (SL-D10P) displayed more elasticity whiles (SL-C80P) showed more viscosity in rheological terms. Finally, (SL-D10P) aggregated in water, detachable by heat and possessed a notable ABTS scavenging ability, thus remarkable potential for biomolecules protection. In conclusion, a better extraction yield of polysaccharides was achieved.
登录
查看更多内容
分级乙醇沉淀红松球果多糖的表征及生物活性
DOI：
10.3390/molecules18089933
发表时间：
2013-08-19
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Zou P;Yang X;Huang WW;Zhao HT;Wang J;Xu RB;Hu XL;Shen SY;Qin D
通讯作者：
Qin D
影响因子：
0.6
作者：
H. P. Ramesh;R. Tharanathan
通讯作者：
H. P. Ramesh;R. Tharanathan
影响因子：
8.4
作者：
Vinatoru, M
通讯作者：
Vinatoru, M
影响因子：
8.4
作者：
Barati, Amir H.;Mokhtarl-Dizaji, Manijhe;Hassan, Zahir M.
通讯作者：
Hassan, Zahir M.
影响因子：
6.2
作者：
Xueqiao Xie;Yun Wang;Juan Han;Yongsheng Yan
通讯作者：
Xueqiao Xie;Yun Wang;Juan Han;Yongsheng Yan