Wolbachia cifB induces cytoplasmic incompatibility in the malaria mosquito vector.
Wolbachia cifB induces cytoplasmic incompatibility in the malaria mosquito vector.
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DOI：
10.1038/s41564-021-00998-6
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发表时间：
2021-12
影响因子：
28.3
通讯作者：
Catteruccia F
中科院分区：
文献类型：
--
作者：
Adams KL;Abernathy DG;Willett BC;Selland EK;Itoe MA;Catteruccia F
沃尔巴克氏体是一种母系遗传的胞内细菌，可通过细胞质不相容性（CI）操纵宿主昆虫的繁殖，导致感染的雄性和未感染的雌性杂交的胚胎死亡。C1由两个原噬菌体基因cifA和cifB编码。沃尔巴克氏体与昆虫不育技术相结合，已在实地试验中用于控制登革热媒介白纹伊蚊的种群，但不知道CI诱导菌株会感染疟疾媒介冈比亚按蚊。在这里，我们表明，cifA和cifB可以诱导疟疾载体An的条件不育。冈比亚。我们使用转基因表达这些沃尔巴克氏体衍生的基因在An。gambiae生殖系，以表明cifB足以导致胚胎致死，cifB诱导的不育性通过cifA在雌性中的表达而得到拯救。当我们在雄性蚊子中共表达cifA和cifB时，CI表型减弱。在雌性蚊子中，cifB损害生育能力，这是克服了cifA的共表达。我们的研究结果为使用CI控制疟疾蚊子媒介铺平了道路。冈比亚按蚊雄性中的沃尔巴克氏体cifB表达足以诱导对所涉及因子的表达水平敏感的细胞质不相容性。
Wolbachia, a maternally inherited intracellular bacterial species, can manipulate host insect reproduction by cytoplasmic incompatibility (CI), which results in embryo lethality in crosses between infected males and uninfected females. CI is encoded by two prophage genes, cifA and cifB. Wolbachia, coupled with the sterile insect technique, has been used in field trials to control populations of the dengue vector Aedes albopictus, but CI-inducing strains are not known to infect the malaria vector Anopheles gambiae. Here we show that cifA and cifB can induce conditional sterility in the malaria vector An. gambiae. We used transgenic expression of these Wolbachia-derived genes in the An. gambiae germline to show that cifB is sufficient to cause embryonic lethality and that cifB-induced sterility is rescued by cifA expression in females. When we co-expressed cifA and cifB in male mosquitoes, the CI phenotype was attenuated. In female mosquitoes, cifB impaired fertility, which was overcome by co-expression of cifA. Our findings pave the way towards using CI to control malaria mosquito vectors. Wolbachia cifB expression in Anopheles gambiae males is sufficient to induce cytoplasmic incompatibility that is sensitive to the expression level of the factors involved.
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作者：
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通讯作者：
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影响因子：
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作者：
Klasson, Lisa;Walker, Thomas;Sebaihia, Mohammed;Sanders, Mandy J.;Quail, Michael A.;Lord, Angela;Sanders, Susanne;Earl, Julie;O'Neill, Scott L.;Thomson, Nicholas;Sinkins, Steven P.;Parkhill, Julian
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