Nitric oxide activatable photosensitizer accompanying extremely elevated two-photon absorption for efficient fluorescence imaging and photodynamic therapy.
Nitric oxide activatable photosensitizer accompanying extremely elevated two-photon absorption for efficient fluorescence imaging and photodynamic therapy.
复制标题
DOI：
10.1039/c7sc04044j
复制
发表时间：
2018-01-28
期刊：
影响因子：
8.4
通讯作者：
Fan Q
中科院分区：
文献类型：
--
作者：
Hu W;Xie M;Zhao H;Tang Y;Yao S;He T;Ye C;Wang Q;Lu X;Huang W;Fan Q
构建了一氧化氮 (NO) 可激活光敏剂，用于高效荧光成像和光动力治疗。一氧化氮 (NO) 水平升高在各种炎症性疾病中发挥重要的病理作用。开发具有双光子激发 (TPE) 功能的 NO 可激活治疗诊断材料对于精密成像和治疗来说非常有前景，但构建此类材料仍然是一个巨大的挑战。在这里，我们提出了第一个 NO 激活荧光光敏剂 (DBB-NO) 的例子，它伴随着 NO 极度升高的双光子吸收 (TPA)，用于有效的荧光成像和光动力治疗 (PDT)。在响应 NO 后，DBB-NO 不仅显示出显着增强的荧光量子产率（ΦF，0.17% vs. 9.3%）和单线态氧量子产率（ΦΔ，1.2% vs. 82%），而且还显示出极大提高的 TPA 截面（δ，270 vs. 2800 GM）。 ΦΔ、ΦF 和 δ 的同时增强可实现前所未有的双光子荧光亮度 (δ × ΦF = 260.4 GM) 和双光子 PDT (TP-PDT) 效率 (δ × ΦΔ = 2296 GM)，这比商用双光子光敏剂的值高出两个数量级。凭借这些优点，NO 可激活的双光子荧光成像和 TP-PDT 在激活的巨噬细胞（其中 NO 过量产生）中的概念验证应用很容易实现。这项工作可能为构建具有其他病理条件可激活特征的双光子治疗诊断材料提供许多机会，以实现精确的治疗诊断。
A nitric oxide (NO) activatable photosensitizer was constructed for efficient fluorescence imaging and photodynamic therapy. Elevated nitric oxide (NO) levels perform an important pathological role in various inflammatory diseases. Developing NO-activatable theranostic materials with a two-photon excitation (TPE) feature is highly promising for precision imaging and therapy, but constructing such materials is still a tremendous challenge. Here, we present the first example of a NO-activatable fluorescent photosensitizer (DBB-NO) accompanying extremely NO-elevated two-photon absorption (TPA) for efficient fluorescence imaging and photodynamic therapy (PDT). Upon responding to NO, DBB-NO shows not only a remarkably enhanced fluorescence quantum yield (ΦF, 0.17% vs. 9.3%) and singlet oxygen quantum yield (ΦΔ, 1.2% vs. 82%) but also an extremely elevated TPA cross-section (δ, 270 vs. 2800 GM). Simultaneous enhancement of ΦΔ, ΦF and δ allows unprecedented two-photon fluorescence brightness (δ × ΦF = 260.4 GM) and two-photon PDT (TP-PDT) efficiency (δ × ΦΔ = 2296 GM) which precedes the value for a commercial two-photon photosensitizer by two orders of magnitude. With these merits, the proof-of-concept applications of NO-activatable two-photon fluorescence imaging and TP-PDT in activated macrophages (in which NO is overproduced) were readily realized. This work may open up many opportunities for constructing two-photon theranostic materials with other pathological condition-activatable features for precise theranostics.
登录
查看更多内容
高度特异性的 C-C 键裂解诱导 FRET 荧光，用于体内生物一氧化氮成像。
影响因子：
8.4
作者：
Li H;Zhang D;Gao M;Huang L;Tang L;Li Z;Chen X;Zhang X
通讯作者：
Zhang X
影响因子：
7.8
作者：
Oberhammer, F A;Hochegger, K;Froschl, G;Tiefenbacher, R;Pavelka, M
通讯作者：
Pavelka, M
通过原位非线性光子转换进行光动力治疗
影响因子：
35
作者：
Kachynski, A. V.;Pliss, A.;Prasad, P. N.
通讯作者：
Prasad, P. N.
影响因子：
5.5
作者：
Fan, QL;Zhou, Y;Huang, W
通讯作者：
Huang, W
高电荷钌(II)聚吡啶配合物作为溶酶体定位光敏剂用于双光子光动力疗法
影响因子：
16.6
作者：
Huang, Huaiyi;Yu, Bole;Chao, Hui
通讯作者：
Chao, Hui