Measuring the frequency and distribution of meiotic crossovers in homozygous barley inbred lines.
Measuring the frequency and distribution of meiotic crossovers in homozygous barley inbred lines.
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DOI：
10.3389/fpls.2022.965217
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
我们报道了一种新的方法，通过结合低水平的EMS突变、快速育种、全基因组鸟枪测序和诱导分子变异数据的滑动窗口分析来确定单个纯合近交系中CO事件的数量和位置。我们通过探索自交大麦品种Bowman自交后代中CO的频率和分布来演示这种方法，并将这些观察到的数据与包含DNA错配修复基因HvMLH3突变的Bowman近等基因系(BW230 Hvmlh3)获得的类似数据进行比较。我们之前已经证明，Hvmlh3使植物的育性和重组都降低了~50%。我们将我们的结果与以前发表的对来自含有WT或HvMLH3突变等位基因的多个F2系的F3家族的传统遗传分析的结果进行了比较，揭示了分析之间的高度对应。我们讨论了该方法在简化植物减数分裂研究中重组评估的可能应用。
We report a novel approach for establishing the number and position of CO events in individual homozygous inbred plants by combining low level EMS mutagenesis, speed breeding, whole genome shotgun sequencing and sliding window analysis of the induced molecular variant data. We demonstrate the approach by exploring CO frequency and distribution in self-fertilised progeny of the inbred barley cultivar Bowman and compare these observations to similar data obtained from a Bowman nearly isogenic line (BW230 Hvmlh3) containing a mutation in the DNA mismatch repair gene HvMLH3. We have previously shown that Hvmlh3 decreases both plant fertility and recombination by ~50%. We compare our results to those from previously published traditional genetic analysis of F3 families derived from multiple F2 lines containing WT or mutant alleles of HvMLH3, revealing a high level of correspondence between analyses. We discuss possible applications of the approach in streamlining the assessment of recombination in plant meiosis research.
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影响因子：
5.6
作者：
Blary A;Gonzalo A;Eber F;Bérard A;Bergès H;Bessoltane N;Charif D;Charpentier C;Cromer L;Fourment J;Genevriez C;Le Paslier MC;Lodé M;Lucas MO;Nesi N;Lloyd A;Chèvre AM;Jenczewski E
通讯作者：
Jenczewski E
影响因子：
1.2
作者：
Cingolani, Pablo;Platts, Adrian;Ruden, Douglas M.
通讯作者：
Ruden, Douglas M.
使用下一代 DNA 测序数据进行变异发现和基因分型的框架。
影响因子：
30.8
作者：
通讯作者：
--
影响因子：
5.6
作者：
Arrieta M;Macaulay M;Colas I;Schreiber M;Shaw PD;Waugh R;Ramsay L
通讯作者：
Ramsay L
影响因子：
5.8
作者：
Choi, Yongwook;Chan, Agnes P.
通讯作者：
Chan, Agnes P.