Transcriptome analysis of carbohydrate metabolism during bulblet formation and development in Lilium davidii var. unicolor.
Transcriptome analysis of carbohydrate metabolism during bulblet formation and development in Lilium davidii var. unicolor.
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百合小球形成和发育过程中碳水化合物代谢的转录组分析。
DOI：
10.1186/s12870-014-0358-4
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发表时间：
2014-12-19
影响因子：
5.3
通讯作者：
Sun H
中科院分区：
文献类型：
--
作者：
Li X;Wang C;Cheng J;Zhang J;da Silva JA;Liu X;Duan X;Li T;Sun H
小鳞茎的形成和发育对于百合属至关重要，因为这些过程与碳水化合物代谢密切相关，特别是与淀粉和蔗糖代谢密切相关。然而，人们对这两个过程的转录调控知之甚少。为了深入了解参与鳞茎形成和发育的碳水化合物相关基因，我们对百合进行了比较转录组分析。鳞片繁殖后0 d、15 d（球茎出现）和35 d（球茎形成具有三至四鳞片的基本形状）的单色鳞茎。转录组分析显示，总共生成了 52,901 个单基因，平均序列大小为 630 bp。根据直系同源群簇 (COG) 分析，8% 的序列归因于碳水化合物运输和代谢。 KEGG通路富集分析结果显示，淀粉和蔗糖代谢是三个文库对中的主导通路。母鳞和鳞茎中淀粉含量分别降低和升高，其变化趋势与蔗糖含量基本一致。对淀粉和蔗糖代谢关键酶的基因表达分析表明，蔗糖合酶（SuSy）和转化酶（INV）主要水解蔗糖，在鳞茎出现和膨大阶段的母鳞和鳞茎中基因表达较高，而蔗糖磷酸合酶（SPS）在形态发生阶段在鳞茎中表达较高。参与淀粉合成方向的酶如ADPG焦磷酸化酶(AGPase)、可溶性淀粉合酶(SSS)、淀粉分支酶(SBE)和颗粒结合淀粉合酶(GBSS)在鳞茎出现和膨大阶段表现出母鳞减少的趋势和较高的基因表达量，而在卵裂阶段则表现出较高的基因表达量 方向，淀粉脱支酶（SDBE），在母鳞中表现出比鳞茎更高的基因表达。对三个鳞茎发育阶段的广泛转录组分析为我们在转录水平上理解百合中碳水化合物代谢相关基因提供了大量新信息，并在分子水平上证明了碳水化合物代谢在鳞茎出现和发育中的基础性。这可以促进进一步研究百合和其他相关物种这些过程背后的分子机制。本文的在线版本 (doi:10.1186/s12870-014-0358-4) 包含补充材料，可供授权用户使用。
The formation and development of bulblets are crucial to the Lilium genus since these processes are closely related to carbohydrate metabolism, especially to starch and sucrose metabolism. However, little is known about the transcriptional regulation of both processes. To gain insight into carbohydrate-related genes involved in bulblet formation and development, we conducted comparative transcriptome profiling of Lilium davidii var. unicolor bulblets at 0 d, 15 d (bulblets emerged) and 35 d (bulblets formed a basic shape with three or four scales) after scale propagation. Analysis of the transcriptome revealed that a total of 52,901 unigenes with an average sequence size of 630 bp were generated. Based on Clusters of Orthologous Groups (COG) analysis, 8% of the sequences were attributed to carbohydrate transport and metabolism. The results of KEGG pathway enrichment analysis showed that starch and sucrose metabolism constituted the predominant pathway among the three library pairs. The starch content in mother scales and bulblets decreased and increased, respectively, with almost the same trend as sucrose content. Gene expression analysis of the key enzymes in starch and sucrose metabolism suggested that sucrose synthase (SuSy) and invertase (INV), mainly hydrolyzing sucrose, presented higher gene expression in mother scales and bulblets at stages of bulblet appearance and enlargement, while sucrose phosphate synthase (SPS) showed higher expression in bulblets at morphogenesis. The enzymes involved in the starch synthetic direction such as ADPG pyrophosphorylase (AGPase), soluble starch synthase (SSS), starch branching enzyme (SBE) and granule-bound starch synthase (GBSS) showed a decreasing trend in mother scales and higher gene expression in bulblets at bulblet appearance and enlargement stages while the enzyme in the cleavage direction, starch de-branching enzyme (SDBE), showed higher gene expression in mother scales than in bulblets. An extensive transcriptome analysis of three bulblet development stages contributes considerable novel information to our understanding of carbohydrate metabolism-related genes in Lilium at the transcriptional level, and demonstrates the fundamentality of carbohydrate metabolism in bulblet emergence and development at the molecular level. This could facilitate further investigation into the molecular mechanisms underlying these processes in lily and other related species. The online version of this article (doi:10.1186/s12870-014-0358-4) contains supplementary material, which is available to authorized users.
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影响因子：
2.6
作者：
Liu, Xiaomei;Yang, Guochen
通讯作者：
Yang, Guochen
影响因子：
6.2
作者：
Okada, Takashi;Singh, Mohan B.;Bhalla, Prem L.
通讯作者：
Bhalla, Prem L.
影响因子：
4.3
作者：
Castro, Antonio J.;Clement, Christophe
通讯作者：
Clement, Christophe
影响因子：
4.3
作者：
He, Xiu-li;Shi, Li-wei;Yi, Ming-fang
通讯作者：
Yi, Ming-fang
影响因子：
5.2
作者：
Ibanez, Ana M.;Martinelli, Federico;Dandekara, Abhaya M.
通讯作者：
Dandekara, Abhaya M.